Lo Wen细胞
运输方式： 冻存运输
生长状态： 贴壁生长
是否是肿瘤细胞： 0
物种来源： 人
ATCC Number： CRL-1159™
相关**： 着色性干皮病
年限： 60 years
数量： 大量
器官来源： 皮肤
Lo Wen细胞细胞形态： 成纤维样
Designations: Lo Wen
Biosafety Level: 1
Shipped: frozen
Medium & Serum: See Propagation
Growth Properties: adherent
Organism: Homo sapiens
Morphology: fibroblast
Source: Organ: skin
Disease: Lo Wen细胞xeroderma pigmentosum, presumed heterozygote
Permits/Forms: In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
Age: 60 years
Gender: female
Comments: The tissue donor was a presumed heterozygote for xeroderma pigmentosum.
Propagation: ATCC complete growth medium: The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium, Catalog No. 30-2002. Lo Wen细胞To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Subculturing: Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:4 is recommended
Medium Renewal: 2 to 3 times per week
Remove spent medium, add fresh 0.25% trypsin for 1 to 2 minutes, remove trypsin and let the culture sit at room temperature for 5 to 10 minutes. Add fresh medium, aspirate and dispense into new flasks. Lo Wen细胞Subculture every 6 to 8 days.
