G26.4.1C3/86 [RAT 1C3/86]细胞
运输方式： 冻存运输
生长状态： 悬浮生长
数量： 大量
细胞形态： **样
**类型： IgG1
是否是肿瘤细胞： 0
物种来源： 小鼠
ATCC Number： HB-9893™
G26.4.1C3/86 [RAT 1C3/86]细胞Designations: G26.4.1C3/86 [RAT 1C3/86]
Depositors: Agen Biomedical, Ltd.
Isotype: IgG1
Biosafety Level: 1
Shipped: frozen
Medium & Serum: See Propagation
Growth Properties: suspension
Organism: Mus musculus (B cell); Mus musculus (myeloma) deposited as mouse (B cell); mouse (myeloma)
Morphology: G26.4.1C3/86 [RAT 1C3/86]细胞lymphoblast
Source: Cell Type: hybridoma: B lymphocyte;
Cellular Products: immunoglobulin; monoclonal antibody; against human red blood cell (erythrocyte, RBC) antigen, glycophorin
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Comments: Animals were immunized with human erythrocytes.
Spleen cells were fused with mouse myeloma cells.
The antibody will bind to, but not agglutinate, freshly collected human red blood cells.
Propagation: ATCC complete growth medium: The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, Catalog No. 30-2001. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 15%.
G26.4.1C3/86 [RAT 1C3/86]细胞Temperature: 37.0°C
Subculturing: Medium Renewal: Every 2 to 3 days
Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 X 10 exp5 cells/ml and maintain between 1 X 10 exp5 and 1 X 10 exp6 cells/ml.
Preservation: culture medium 95%; DMSO, 5%
Related Products: Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2001
recommended serum:ATCC 30-2020
References: 3947: Rylatt DB, et al. Erythrocyte agglutination assay. G26.4.1C3/86 [RAT 1C3/86]细胞US Patent 4,894,347 dated Jan 16 1990
