TGP49细胞
生长状态： 贴壁生长
品系： Tg(E1a-1-SV40E)Bri18
年限： 26 weeks
器官来源： 胰腺
是否是肿瘤细胞： 0
物种来源： 转基因小鼠
ATCC Number： CRL-2136™
运输方式： 冻存运输
相关**： 其他**
细胞形态： 上皮样
TGP49细胞数量： 大量
Designations: TGP49
Depositors: OS Pettengill, D Longnecker
Biosafety Level: 2
Shipped: frozen
Medium & Serum: See Propagation
Growth Properties: adherent
Organism: Mus musculus, transgenic deposited as mouse, transgenic
Morphology: epithelial
Source: Organ: pancreas
Strain: Tg(E1a-1-SV40E)Bri18
Disease: pancreatic acinar cell tumor; carcinoma
Permits/Forms: TGP49细胞In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
Restrictions: Note: These cells are distributed subject to the following: 1.) This cell line or its products must not be distributed to third parties. Commercial interests are the exclusive property of Dartmouth College. 2.) Any proposed commercial use of these cells must first be negotiated with Office of Technology Transfer, Dartmouth College, Hanover, NH, 03755. Tel: (603) 646-3675. 3.) In all papers reporting any use of these cells, or derived products, a direct reference will be made to the original publications.
Age: 26 weeks
Gender: male
Comments: TGP49 is an epithelial-like cell line derived from a pancreatic tumor with acinar cell morphology that arose in an ***** male transgenic mouse.
The mouse carried the pseudogene construct composed of elastase-1 promoter linked to the SV40 T antigen.
In culture, TGP49 cells lost acinar cell morphology and transiently produced somatostatin.
Current stocks of TGP49 are negative for somatostatin and positive for trypsin. The cells do not secrete amylase or lipase. Morphologic and differentiation markers of this line appear to fluctuate from time to time in culture.
Propagation: TGP49细胞ATCC complete growth medium: A 1:1 mixture of Dulbecco's modified Eagle's medium and Ham's F12 medium, 90%; heat-inactivated fetal bovine serum, 10%
Subculturing: Subcultivation Ratio: A subcultivation ratio of 1:3 to 1:4 is recommended
Medium Renewal: Twice per week
Remove spent medium, add fresh 0.25% trypsin, 0.03% EDTA solution, rinse and remove trypsin. Let the culture sit at room temperature (or at 37C) until the cells detach. Add fresh medium, aspirate and dispense into new flasks.
Preservation: Culture medium, 95%; DMSO, 5%
References: 22323: Pettengill OS, et al. Cell lines derived from pancreatic tumors of Tg(Ela-1-SV40E)Bri18 transgenic mice express somatostatin and T antigen. Carcinogenesis 15: 61-65, 1994. TGP49细胞PubMed: 7904904
