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G14D细胞

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  • 产品名称:G14D细胞
  • 产品型号:G14D
  • 产品展商:HZbscience
  • 产品文档:无相关文档
  • 发布时间:2018-06-20
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简单介绍
G14D细胞应如何避免细胞污染,细胞污染的种类可分成**、酵母菌、霉菌、病毒和霉浆菌。主要的污染原因为无菌操作技术不当、操作室环境不佳、污染之血清和污染之细胞等。严格之无菌操作技术、清洁的环境、与品质良好之细胞来源和培养基配制是减低污染之*好方法。G14D细胞何时须更换培养基?视细胞生长密度而定,或遵照细胞株基本数据上之更换时间,按时更换培养基即可。
产品描述

G14D细胞

数量: 大量

细胞形态: **样

运输方式: 冻存运输

细胞类型: 其他细胞类型

是否是肿瘤细胞: 0

物种来源: 其他

器官来源: 外周血

生长状态: 悬浮生长,多细胞聚集

品系: Marion

G14D细胞ATCC Number: CRL-2760™

Designations: G14D

Depositors: NW Miller

Biosafety Level: 1

Shipped: frozen

Medium & Serum: See Propagation

Growth Properties: suspension, multicell aggregates

Organism: Ictalurus punctatus

Morphology: lymphoblast


Source: Strain: Marion

Organ: peripheral blood

Cell Type: G14D细胞lymphocyte T lymphocyte;

Permits/Forms: In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.

Isolation: Isolation date: 1998

Receptors: T cell antigen receptor, expressed

Cytogenetic Analysis: diploid

Comments: The G14D cell line developed in 1998 is an immortal homozygous T cell line spontaneously derived by in vitro lipopolysaccharide (LPS) stimulation of peripheral blood from a normal gynogenetic channel catfish.. The cells were, subsequently, cloned by limiting dilution. The cells are maintained in vitro without restimulation, feeder cells, or exogenous factors. The cells constitutively produce interleukin-like factors.

Propagation:G14D细胞 ATCC complete growth medium: A 1:1 mixture of Leibovitz's L-15 medium with 2 mM L-glutamine and AIM-V Medium adjusted to contain 1.5 g/L sodium bicarbonate and supplemented with 0.05 mM 2-mercaptoethanol, 10% double distilled water and 5% heat-inactivated catfish serum

Atmosphere: air, 95%; carbon dioxide (CO2), 5%

Temperature: 27.0°C

Subculturing: Protocol: Cultures can be maintained by the addition of fresh medium or replacement of medium. Alternatively, cultures can be established by centrifugation with subsequent resuspension at 5 X 10(5) viable cells/ml. Maintain cell density between 5 X 10(5) and 5 X 10(6) viable cells/ml.

Medium Renewal: Add fresh medium every 2 to 3 days (depending on cell density)

Preservation: Freeze medium: Fetal Bovine Serum supplented with 7.5% DMSO

Storage temperature: liquid nitrogen vapor phase

References: 61263: Hogan RJ, et al. G14D细胞Molecular and Immunologic Characterization of Gynogenetic Channel Catfish (Ictalurus punctatus). Mar. Biotechnol. 1: 317-327, 1999. PubMed: 10489406

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