146-03E04 [146-3E4]细胞
ATCC Number： CRL-2650™
运输方式： 冻存运输
数量： 大量
是否是肿瘤细胞： 0
物种来源： 小鼠
生长状态： 悬浮生长
细胞形态： **样
**类型： IgG1; kappa light chain
146-03E04 [146-3E4]细胞Designations: 146-03E04 [146-3E4]
Depositors: National Cancer Institute
Isotype: IgG1; kappa light chain
Biosafety Level: 1
Shipped: frozen
Medium & Serum: See Propagation
Growth Properties: suspension, but many cells attach
Organism: Mus musculus (B cell); Mus musculus (myeloma) deposited as mouse (B cell); mouse (myeloma)
Morphology: lymphoblast
Source: Cell Type: hybridoma: B lymphocyte;
Cellular Products:146-03E04 [146-3E4]细胞 immunoglobulin; monoclonal antibody; against a synthetic ras oncogene peptide
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Comments: Animals were immunized with a synthetic polypeptide with the sequence YTLVREIRQHKLRKLNPPDESGPGC corresponding to peptides 157 to 181 of the H-ras oncogene.
Spleen cells were fused with Sp2/0 myeloma cells.
The antibody reacts with the synthetic H-ras oncogene peptide. It reacts with the Harvey (Ha-ras) p21 protein but not the Kirsten (Ki-ras) p21 protein.
Propagation: 146-03E04 [146-3E4]细胞ATCC complete growth medium: The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Temperature: 37.0°C
Subculturing: Medium Renewal: Add fresh medium every 2 to 3 days (depending on cell density)
Cultures can be maintained by the addition of fresh medium or replacement of medium. Alternatively, cultures can be established by centrifugation with subsequent resuspension at 2 X 10 exp5 viable cells/ml.
Maintain cell density between 1 X 10 exp5 and 1 X 10 exp6 viable cells/ml.
Preservation: 146-03E04 [146-3E4]细胞culture medium 95%; DMSO, 5%
Related Products: Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2002
recommended serum:ATCC 30-2020
References: 21967: Niman HL, Lerner RA. Polypeptide-induced monoclonal receptors to protein ligands. US Patent 5,015,571 dated May 14 1991
55710: Pumiglia K, et al. Raf-1 N-terminal sequences necessary for Ras-Raf interaction and signal transduction. Mol. Cell. Biol. 15: 398-406, 1995. PubMed: 7799948
