TFTB1 [3916.120.15]细胞
生长状态： 悬浮生长
数量： 大量
是否是肿瘤细胞： 0
物种来源： 小鼠
运输方式： 冻存运输
ATCC Number： CRL-1759™
细胞形态： **样
**类型： IgG1
TFTB1 [3916.120.15]细胞Designations: TFTB1 [3916.120.15]
Depositors: R Fulton
Isotype: IgG1
Biosafety Level: 1
Shipped: frozen
Medium & Serum: See Propagation
Growth Properties: suspension
Organism: Mus musculus (B cell); Mus musculus (myeloma) deposited as mouse (B cell); mouse (myeloma)
Morphology: lymphoblast
Source: Cell Type: hybridoma: B lymphocyte;
Cellular Products: TFTB1 [3916.120.15]细胞immunoglobulin; monoclonal antibody; against the B chain of the plant toxin ricin
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Comments: Animals were immunized with ricin B chain.
Spleen cells were fused with Sp2/5 myeloma cells (a subclone of Sp2/0-Ag14).
The antibody does not cross-react with the ricin A chain.
TFTB1 [3916.120.15]细胞Tested and found negative for ectromelia virus (mousepox).
Propagation: ATCC complete growth medium: The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Subculturing: Medium Renewal: Every 2 to 4 days
Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 X 10 exp5 cells/ml and maintain between 1 X 10 exp5 and 1 X 10 exp6 cells/ml.
References: 1321: Fulton RJ, et al. TFTB1 [3916.120.15]细胞Purification of ricin A1, A2, and B chains and characterization of their toxicity. J. Biol. Chem. 261: 5314-5319, 1986. PubMed: 3957927
