G-8细胞
生长状态： 贴壁生长
组织来源： skeletal muscle
细胞形态： 成肌细胞
年限： fetus fetus
运输方式： 冻存运输
细胞类型： 其他细胞类型
是否是肿瘤细胞： 0
G-8细胞物种来源： 小鼠
ATCC Number： CRL-1456™
数量： 大量
Designations: G-8
Depositors: J Peacock
Biosafety Level: 1
Shipped: frozen
Medium & Serum: See Propagation
Growth Properties: adherent
Organism: Mus musculus
G-8细胞Morphology: myoblast
Source: Tissue: skeletal muscle
Cell Type: myoblast myoblast;
Cellular Products: myosin
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Receptors: acetylcholine (The cells are highly responsive to acetylcholine.)
Tumorigenic: No
G-8细胞Cytogenetic Analysis: modal number = 66
Age: fetus fetus
Propagation: ATCC complete growth medium: Dulbecco's modified Eagle's medium with 4 mM L-glutamine adjusted to contain 1.5 g/L sodium bicarbonate and 4.5 g/L glucose, 80%; horse serum, 10%; fetal bovine serum, 10%
Temperature: 37.0°C
Subculturing: Protocol: Remove medium, add fresh 0.25% trypsin, 0.53mM EDTA solution for 2 to 5 minutes, add fresh medium, aspirate and dispense into new Bovine Collagen type l (0.03 mg/ml) coated flasks.Differentiation is enhanced by reducing the sera to 2% each.
Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:4 is recommended
Medium Renewal: Twice per week
Doubling Time: 25 to 27 hrs
References: 1063: Christian CN, et al. G-8细胞Synapse formation between two clonal cell lines. Science 196: 995-998, 1977. PubMed: 193191
