LLC-PK1A细胞
ATCC Number： CL-101.1™
相关**： 正常
生长状态： 贴壁生长
数量： 大量
运输方式： 冻存运输
器官来源： 肾脏
是否是肿瘤细胞： 0
物种来源： 猪
Designations: LLC-PK1A
Depositors: Eli Lilly & Co.
LLC-PK1A细胞Biosafety Level: 1
Shipped: frozen
Medium & Serum: See Propagation
Growth Properties: adherent
Organism: Sus scrofa
Morphology:
Source: Organ: kidney
Disease: normal
Cellular Products: plasminogen activator
Permits/Forms: In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. LLC-PK1A细胞Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
Propagation: ATCC complete growth medium: The base medium for this cell line is Medium 199 with Earle's BSS with 1.5 g/L to 2.2 g/L sodium bicarbonateTo make the complete growth medium, add the following components to the base medium:
5% fetal bovine serum
Temperature: 37.0°C
Atmosphere: air, 95%; carbon dioxide (CO2), 5%
Subculturing: Subcultivation Ratio: A subcultivation ratio of 1:4 to 1:8 is recommended
Medium Renewal: Twice per week
Protocol: Volumes used in this protocol are for 75 sq cm flasks; proportionally reduce or increase amount of dissociation medium for culture vessels of other sizes.
Remove and discard culture medium.
LLC-PK1A细胞Briefly rinse the cell layer with Ca++/Mg++ free Dulbecco's phosphate-buffered saline (D-PBS) or 0.25% (w/v) Trypsin - 0.53 mM EDTA solution to remove all traces of serum which contains trypsin inhibitor.
Add 2.0 to 3.0 ml of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).
Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37C to facilitate dispersal.
Add 2.0 to 3.0 ml of complete growth medium and aspirate cells by gently pipetting
Resuspend the cell pellet in fresh growth medium. Add appropriate aliquots of the cell suspension to new culture vessels.
Incubate cultures at 37C.
Preservation: culture medium, 90%; additional serum, 5%; DMSO, 5%
References: 3520: Hull RN, Huseby RM. LLC-PK1A细胞Enhanced production of plasminogen activator. US Patent 3,904,480 dated Sep 9 1975
