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LLC-PK1A细胞

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  • 产品名称:LLC-PK1A细胞
  • 产品型号:LLC-PK1A
  • 产品展商:HZbscience
  • 产品文档:无相关文档
  • 发布时间:2018-06-26
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简单介绍
LLC-PK1A细胞应如何避免细胞污染,细胞污染的种类可分成**、酵母菌、霉菌、病毒和霉浆菌。主要的污染原因为无菌操作技术不当、操作室环境不佳、污染之血清和污染之细胞等。严格之无菌操作技术、清洁的环境、与品质良好之细胞来源和培养基配制是减低污染之*好方法。LLC-PK1A细胞何时须更换培养基?视细胞生长密度而定,或遵照细胞株基本数据上之更换时间,按时更换培养基即可。
产品描述

LLC-PK1A细胞

ATCC Number: CL-101.1™

相关**: 正常

生长状态: 贴壁生长

数量: 大量

运输方式: 冻存运输

器官来源: 肾脏

是否是肿瘤细胞: 0

物种来源: 猪

Designations: LLC-PK1A

Depositors: Eli Lilly & Co.

LLC-PK1A细胞Biosafety Level: 1

Shipped: frozen

Medium & Serum: See Propagation

Growth Properties: adherent

Organism: Sus scrofa

Morphology:

Source: Organ: kidney

Disease: normal

Cellular Products: plasminogen activator

Permits/Forms: In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. LLC-PK1A细胞Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.

Propagation: ATCC complete growth medium: The base medium for this cell line is Medium 199 with Earle's BSS with 1.5 g/L to 2.2 g/L sodium bicarbonateTo make the complete growth medium, add the following components to the base medium:

5% fetal bovine serum


Temperature: 37.0°C

Atmosphere: air, 95%; carbon dioxide (CO2), 5%

Subculturing: Subcultivation Ratio: A subcultivation ratio of 1:4 to 1:8 is recommended

Medium Renewal: Twice per week

Protocol: Volumes used in this protocol are for 75 sq cm flasks; proportionally reduce or increase amount of dissociation medium for culture vessels of other sizes.

Remove and discard culture medium.

LLC-PK1A细胞Briefly rinse the cell layer with Ca++/Mg++ free Dulbecco's phosphate-buffered saline (D-PBS) or 0.25% (w/v) Trypsin - 0.53 mM EDTA solution to remove all traces of serum which contains trypsin inhibitor.

Add 2.0 to 3.0 ml of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).

Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37C to facilitate dispersal.

Add 2.0 to 3.0 ml of complete growth medium and aspirate cells by gently pipetting

Resuspend the cell pellet in fresh growth medium. Add appropriate aliquots of the cell suspension to new culture vessels.

Incubate cultures at 37C.

Preservation: culture medium, 90%; additional serum, 5%; DMSO, 5%

References: 3520: Hull RN, Huseby RM. LLC-PK1A细胞Enhanced production of plasminogen activator. US Patent 3,904,480 dated Sep 9 1975

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