130C3/2B/8细胞
生长状态： 悬浮生长
是否是肿瘤细胞： 0
物种来源： 小鼠
细胞形态： **样
**类型： IgG1
ATCC Number： HB-8088™
130C3/2B/8细胞运输方式： 冻存运输
数量： 大量
Designations: 130C3/2B/8
Depositors: Johns Hopkins University
Isotype: IgG1
Biosafety Level: 1
Shipped: frozen
Medium & Serum: See Propagation
130C3/2B/8细胞Growth Properties: suspension
Organism: Mus musculus (B cell); Mus musculus (myeloma) deposited as mouse (B cell); mouse (myeloma)
Morphology: lymphoblast
Source: Cell Type: hybridoma: B lymphocyte;
Cellular Products: immunoglobulin; monoclonal antibody; against surface (cercariae) glycoprotein on Schistosoma mansoni
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Comments: 130C3/2B/8细胞Animals were immunized with cercariae glycoprotein from Schistosoma mansoni.
Spleen cells were fused with P3X63Ag8 myeloma cells.
Propagation: ATCC complete growth medium: Dulbecco's modified Eagle's medium with 4.5 g/L glucose, 0.2 units/ml insulin, 3 mM L-glutamine, 1 mM sodium pyruvate, 0.15 mg/ml oxaloacetic acid and 0.01 M HEPES, 80%; NCTC 135 medium, 10%; fetal bovine serum, 10%
Subculturing: Medium Renewal: Every 2 to 3 days
Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 X 10 exp5 cells/ml and maintain between 1 X 10 exp5 and 1 X 10 exp6 cells/ml.
Preservation: culture medium, 40%; fetal bovine serum, 50%; DMSO, 10%
Related Products: Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 46-X
References: 3511: Strand M. Diagnosis and treatment of fluke infections with monoclonal antibodies. 130C3/2B/8细胞US Patent 4,416,866 dated Nov 22 1983
