Aw3.18.14细胞
生长状态： 悬浮生长，少量贴壁
器官来源： 脾
数量： 大量
是否是肿瘤细胞： 0
物种来源： 小鼠
ATCC Number： CRL-2826™
运输方式： 冻存运输
品系： CAF1
细胞形态： **样
**类型： mouse IgG1 kappa
Aw3.18.14细胞Designations: Aw3.18.14
Depositors: ER Unanue
Isotype: mouse IgG1 kappa
Biosafety Level: 1
Shipped: frozen
Medium & Serum: See Propagation
Growth Properties: suspension, with some loosely adherent cells
Organism: Mus musculus (B cell); Mus musculus (myeloma)
Morphology: lymphoblast
Source: Organ: spleen
Strain: CAF1
Cell Type: hybridoma: B lymphocyte; somatic cell hybrid
Permits/Forms: In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Aw3.18.14细胞Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
Isolation: Isolation date: 1996
Comments: Animals were immunized with M12-I-Ak 48-62 cells [PubMed: 9208845]. Spleen cells were fused with P3X63Ag8 myeloma cells.The antibody recognizes peptide residues 48-62 of hen egg lysozyme (HEL) bound to the MHC class II molecule I-Ak.
Propagation: ATCC complete growth medium: Dulbecco's Modified Eagle's Medium with 4 mM L-glutamine that is modified by ATCC to contain 4.5 g/L glucose and 1.5 g/L sodium bicarbonates supplemented with 1.5 mM L-glutamine, 0.05 mM 2-mercaptoethanol, 10 mM HEPES, 116mg/L L-arginine HCl, 41 mg/L L-asparagine.H2O and 10% heat-inactivated fetal bovine serum
Temperature: 37.0°C
Subculturing:Aw3.18.14细胞 Protocol: Cultures can be maintained by the addition of fresh medium or replacement of medium. Shake off the attached cells and transfer along with the floating cells into new flasks. Alternatively, cultures can be established by centrifugation with subsequent resuspension at 1 to 3 X 10(4) viable cells/ml.
Interval: Maintain cell density between 2 X 10(4) and 2 X 10(5) viable cells/ml.
Medium Renewal: Add fresh medium every 2 to 3 days (depending on cell density)
Preservation: Freeze medium: Complete growth medium supplemented with an additional 10% heat-inactivated fetal bovine serum and 7.5% DMSO
Storage temperature: liquid nitrogen vapor phase
Doubling Time: 14 hours
Related Products: Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2002
References: 30557: Dadaglio G, et al. Aw3.18.14细胞Characterization and quantitation of peptide-MHC complexes produced from hen egg lysozyme using a monoclonal antibody. Immunity 6: 727-738, 1997. PubMed: 9208845
