上海沪震实业有限公司
扫一扫,手机逛起来
主营:ELISA试剂盒,人ELISA试剂盒,小鼠ELISA试剂盒,大鼠ELISA试剂盒,重组蛋白,金标试剂盒,**组化试剂盒,标准品,单克隆抗体,多克隆抗体,生化试剂,生物培养基,原代细胞,细胞株,实验室耗材,动物血清等产品
021-60345367
您现在的位置:
企业信息
9
  • 注册时间:2015-11-06
  • 联系人:游艳
  • 电话:021-60345367
  • 联系时,请说明仪表网看到的
  • Email:shhzsw01@163.com
在线询价
产品详情

1D3细胞

  • 如果您对该产品感兴趣的话,可以
  • 产品名称:1D3细胞
  • 产品型号:1D3
  • 产品展商:HZbscience
  • 产品文档:无相关文档
  • 发布时间:2018-07-03
  • 在线询价
简单介绍
1D3细胞应如何避免细胞污染,细胞污染的种类可分成**、酵母菌、霉菌、病毒和霉浆菌。主要的污染原因为无菌操作技术不当、操作室环境不佳、污染之血清和污染之细胞等。严格之无菌操作技术、清洁的环境、与品质良好之细胞来源和培养基配制是减低污染之*好方法。1D3细胞何时须更换培养基?视细胞生长密度而定,或遵照细胞株基本数据上之更换时间,按时更换培养基即可。
产品描述

1D3细胞

生长状态: 悬浮生长

ATCC Number: HB-305™

细胞形态: **样

**类型: IgG2a; kappa light chain

运输方式: 冻存运输

数量: 大量

是否是肿瘤细胞: 0

物种来源: 褐鼠

1D3细胞Designations: 1D3

Depositors: DT Fearon, I Krop, MS Schlissel, AR de Fougerolles

Isotype: IgG2a; kappa light chain

Biosafety Level: 1

Shipped: frozen

Medium & Serum: See Propagation

Growth Properties: suspension

Organism: Rattus norvegicus (B cell); Mus musculus (myeloma)

Morphology: lymphoblast


Source: Cell Type: hybridoma: B lymphocyte;

Cellular Products: 1D3细胞immunoglobulin; monoclonal antibody; against mouse CD19

Permits/Forms: In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.

Comments: The hybridoma cell line 1D3 secretes a rat monoclonal antibody that reacts with the mouse pan-B cell marker CD19.

The line was produced by fusing NS-1 myeloma cells with spleen cells from Lewis rats that had been immunized with transfected K562 cells expressing the extracellular domain of murine CD19.

The antibody binds to mouse CD19 with high affinity, but does not react with human CD19.

It is useful flow cytometric analysis of total mouse B cells and for studies of signal transduction involving CD19.

Propagation: 1D3细胞ATCC complete growth medium: RPMI 1640 medium with 2 mM L-glutamine adjusted to contain1.5 g/L sodium bicarbonate, 4.5 g/L glucose, 10 mM HEPES, and 1.0 mM sodium pyruvate supplemented with 0.05 mM 2-mercaptoethanol, 90%; fetal bovine serum, 10%

Temperature: 37.0°C

Subculturing: Medium Renewal: Every 2 to 3 days

Cultures can be maintained by addition or replacement of fresh medium. Alternately, cultures can be established by centrifugation with subsequent resuspension in fresh culture medium.

Establish new cultures at 2 X 10 exp5 viable cells/ml and maintain at between 1 X 10 exp5 and 1 X 10 exp6 viable cells/ml.

Preservation: Culture medium, 95%; DMSO, 5%

References: 34490: Krop I, et al. 1D3细胞Self-renewal of B-1 lymphocytes is dependent on CD19. Eur. J. Immunol. 26: 238-242, 1996. PubMed: 8566073

关于我们| 易展动态| 易展荣誉| 易展服务| 易家文化| 英才集结号| 社会责任| 联系我们

备案号:粤ICP备11010883号| 公安机关备案号:44040202000312| 版权问题及信息删除: 0756-2183610  QQ: 服务QQ

Copyright?2004-2017  珠海市金信桥网络科技有限公司 版权所有

行业网站百强奖牌 搜索营销*有价值奖 中小企业电子商务**服务商
以上信息由企业自行提供,信息内容的真实性、准确性和合法性由相关企业负责,易展仪表展览网对此不承担任何保证责任。

沪公网安备 31011702004356号