1G1 [LS141-1G1-65-15-1]细胞
生长状态： 悬浮生长
ATCC Number： HB-12624™
数量： 大量
细胞形态： **样
**类型： IgG1 kappa
是否是肿瘤细胞： 0
物种来源： 小鼠
运输方式： 冻存运输
Designations: 1G1 [LS141-1G1-65-15-1]
Depositors: LeukoSite, Inc.
1G1 [LS141-1G1-65-15-1]细胞Isotype: IgG1 kappa
Biosafety Level: 1
Shipped: frozen
Medium & Serum: See Propagation
Growth Properties: suspension
Organism: Mus musculus (B cell); Mus musculus (myeloma)
Morphology: lymphoblast
Source: Cell Type: hybridoma:lymphoblast B lymphocyte; somatic cell hybrid
Permits/Forms: In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
Comments: 1G1 [LS141-1G1-65-15-1]细胞Animals were immunized with L1.2 cells expressing high levels of transfected CCR4.Spleen cells were fused with Sp2/0 myeloma cells.The cells produce immunoglobulin; monoclonal antibody; against human Chemokine Receptor 4 (CCR4).
Propagation: ATCC complete growth medium: Temperature: 37.0°C
Subculturing: Protocol: Cultures can be maintained by the addition of fresh medium or replacement of medium. Alternatively, cultures can be established by centrifugation with subsequent resuspension at 3 X 10(4) viable cells/ml. Maintain cell density between 4 X 10(4) and 4 X 10(5) viable cells/ml. Do not exceed 7 X10(5) cells/ ml.
Medium Renewal: Add fresh medium every 2 to 3 days (depending on cell density)
Preservation: Freeze medium: Complete growth medium, extra 10% FBS; 7.5% DMSO
Storage temperature: liquid nitrogen vapor phase
Doubling Time: 19 hours
Related Products: Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2002
recommended serum:ATCC 30-2020
1G1 [LS141-1G1-65-15-1]细胞References: 71341: Wu L, et al. Anti-CCR4 antibodies and methods of use therefor. US Patent 6,488,930 dated Dec 3 2002
71429: Wilbanks A, et al. Expression cloning of the STRL33/BONZO/TYMSTRligand reveals elements of CC, CXC, and CX3C chemokines. J. Immunol. 188: 5145-5154, 2001. PubMed: 11290797
88727: Jones D, et al. Expression pattern of T-cell-associated chemokine receptors and their chemokines correlates with specific subtypes of T-cell non-Hodgkin lymphoma. Blood 96: 685-690, 2000. PubMed: 10887135
88932: Kim CH, et al. Rules of chemokine receptor association with T cell polarization in vivo. J. Clin. Invest. 108: 1331-1339, 2001. PubMed: 11696578
88933: Campbell JJ, et al. 1G1 [LS141-1G1-65-15-1]细胞The chemokine receptor CCR4 in vascular recognition by cutaneous but not intestinal memory T cells. Nature 400: 776-780, 1999. PubMed: 10466728
