LL 29 (AnHa)细胞
数量： 大量
细胞形态： 成纤维样
生长状态： 贴壁生长
年限： 26 years
ATCC Number： CCL-134™
相关**： 其他**
细胞类型： 成纤维细胞
LL 29 (AnHa)细胞是否是肿瘤细胞： 0
物种来源： 人
运输方式： 冻存运输
器官来源： 肺
Designations: LL 29 (AnHa)
Depositors: RG Crystal
Biosafety Level: 1
Shipped: frozen
Medium & Serum: See Propagation
Growth Properties: adherent
Organism: LL 29 (AnHa)细胞Homo sapiens
Morphology: fibroblast
Source: Organ: lung
Disease: idiopathic pulmonary fibrosis
Cell Type: fibroblast
Permits/Forms: In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
Age: 26 years
LL 29 (AnHa)细胞Gender: female
Ethnicity: Caucasian
Propagation: ATCC complete growth medium: Ham's F12K medium, 85%; fetal bovine serum, 15%
Subculturing: Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:3 is recommended
Medium Renewal: 2 to 3 times per week
Remove medium and add fresh 0.25% trypsin, 0.03% EDTA for 1 to 2 minutes. Remove trypsin, EDTA and incubate the culture at 37C for 10 to 20 minutes with a small volume of trypsin - EDTA. LL 29 (AnHa)细胞Add fresh medium, aspirate and dispense into new flasks.
Preservation: Culture medium, 95%; DMSO, 5%
