LL 86 (LeSa)细胞
器官来源： 肺
细胞形态： 成纤维样
生长状态： 贴壁生长
数量： 大量
细胞类型： 成纤维细胞
是否是肿瘤细胞： 0
物种来源： 人
年限： 18 years
ATCC Number： CCL-190™
LL 86 (LeSa)细胞相关**： 正常
运输方式： 冻存运输
Designations: LL 86 (LeSa)
Depositors: RG Crystal
Biosafety Level: 1
Shipped: frozen
Medium & Serum: See Propagation
Growth Properties: adherent
Organism: Homo sapiens
Morphology: fibroblast
LL 86 (LeSa)细胞Source: Organ: lung
Disease: normal
Cell Type: fibroblast
Permits/Forms: In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
Cytogenetic Analysis: normal human male; diploid
Isoenzymes: G6PD, B
Age: 18 years
LL 86 (LeSa)细胞Gender: male
Ethnicity: Caucasian
Comments: The line was derived from tissue normal tissue from a patient with osteogenic sarcoma.
Propagation: ATCC complete growth medium: Ham's F12K medium, 85%; fetal bovine serum, 15%
Subculturing: Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:4 is recommended
Medium Renewal: 2 to 3 times per week
LL 86 (LeSa)细胞Remove medium, rinse the monolayer once with fresh 0.25% trypsin and let the culture sit for 10 to 15 minutes at room temperature. Add fresh medium, aspirate and dispense into new flasks.
