TE15细胞
细胞形态： **样
**类型： IgM
数量： 大量
是否是肿瘤细胞： 0
物种来源： 小鼠
生长状态： 悬浮生长
ATCC Number： HB-206™
运输方式： 冻存运输
Designations: TE15
TE15细胞Depositors: BF Haynes
Isotype: IgM
Biosafety Level: 1
Shipped: frozen
Medium & Serum: See Propagation
Growth Properties: suspension
Organism: Mus musculus (B cell); Mus musculus (myeloma) deposited as mouse (B cell); mouse (myeloma)
Morphology: lymphoblast
Source: Cell Type: hybridoma: B lymphocyte;
Cellular Products: immunoglobulin; monoclonal antibody; against human thymic epithelium (Hassall's bodies)
Permits/Forms: TE15细胞In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
Comments: Animals were immunized with human thymic stroma.
Spleen cells were fused with NS-1 myeloma cells.
The antibody reacts with granular foci among the cellular swirls of Hassall's bodies.
It defines an antigen that is acquired between 14 and 16 weeks of fetal gestation, and marks the terminal stages of epidermal keratinocyte maturation.
TE15细胞The antibody binds specifically to granular foci among the cellular swirls of most Hassall's bodies and to stratum corneum of skin epidermis and foreskin.
Tested and found negative for ectromelia virus (mousepox).
Propagation: ATCC complete growth medium: Dulbecco's modified Eagle's medium with 4.5 g/L glucose, 80%; heat-inactivated fetal bovine serum, 20%
Subculturing: Medium Renewal: Every 2 to 3 days
Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 X 10 exp5 cells/ml and maintain between 1 X 10 exp5 and 1 X 10 exp6 cells/ml.
References: 1637: Lobach DF, et al. TE15细胞The human thymic microenvironment. Phenotypic characterization of Hassall's bodies with the use of monoclonal antibodies. J. Immunol. 134: 250-257, 1985. PubMed: 2578044
