1F5细胞
细胞形态： **样
**类型： IgG2a
生长状态： 悬浮生长
是否是肿瘤细胞： 0
物种来源： 小鼠
ATCC Number： HB-9645™
数量： 大量
运输方式： 冻存运输
1F5细胞Designations: 1F5
Depositors: Bristol-Myers Co.
Isotype: IgG2a
Biosafety Level: 1
Shipped: frozen
Medium & Serum: See Propagation
Growth Properties: suspension
Organism: Mus musculus (B cell); Mus musculus (myeloma) deposited as mouse (B cell); mouse (myeloma)
Morphology: lymphoblast
Source: Cell Type: 1F5细胞hybridoma: B lymphocyte;
Cellular Products: immunoglobulin; monoclonal antibody; against human CD20 B cell antigen (Bp35)
Permits/Forms: In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
Comments: The antibody is specific for CD20 (expressed on normal and neoplastic human B cells) and can induce proliferation of resting B cells.
Propagation: ATCC complete growth medium: The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, Catalog No. 30-2001. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
1F5细胞Temperature: 37.0°C
Subculturing: Medium Renewal: Every 2 to 3 days
Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 X 10 exp5 cells/ml and maintain between 1 X 10 exp5 and 1 X 10 exp6 cells/ml.
References: 21956: Senter PD, et al. Antibody-enzyme conjugates in combination with prodrugs for the delivery of cytotoxic agents to tumor cells. US Patent 4,975,278 dated Dec 4 1990
23287: Clark EA, et al. 1F5细胞Role of the Bp35 cell surface polypeptide in human B-cell activation. Proc. Natl. Acad. Sci. USA 82: 1766-1770, 1985. PubMed: 3872456
