AT3B-1细胞
器官来源： 前列腺
细胞形态： 上皮样
是否是肿瘤细胞： 0
物种来源： 褐鼠
年限： 22 months
运输方式： 冻存运输
数量： 大量
品系： Copenhagen
ATCC Number： CRL-2375™
AT3B-1细胞相关**： 其他**
Designations: AT3B-1
Depositors: KJ Pienta
Biosafety Level: 1
Shipped: frozen
Medium & Serum: See Propagation
Organism: Rattus norvegicus deposited as rat
Morphology: epithelial
Source: Organ: prostate
Strain: Copenhagen
Disease: AT3B-1细胞malignant carcinoma
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Age: 22 months
Gender: male
Comments: AT3B-1 was derived from the AT-3 cell line. AT-3 was derived from a ***** malignant rat prostate tumor obtained from a 22 month old inbred Copenhagen rat by Isaacs et al. [39920]
The parental cells were grown in increasing concentrations of the drug doxorubicin until the final concentration of 0.001 mM was achieved.
These cells express a multidrug (MDR) resistant phenotype. They are more resistant to vinblastine compared to controls.
When P-glycoprotein was blocked, the AT3 B-1 cell line demonstrated drug efflux pump activity. Injection of AT3 B-1 cells into rats followed by doxorubicin treatment produced larger tumors compared to the parental controls. [39921]
AT3B-1细胞This cell line can be used to study chemotherapy resistance in prostate cancer.
Propagation: ATCC complete growth medium: RPMI 1640 medium with 2 mM L-glutamine adjusted to contain 1.5 g/L sodium bicarbonate, 4.5 g/L glucose, 10 mM HEPES, and 1.0 mM sodium pyruvate and supplemented with 0.001 mM doxorubicin, 90%; fetal bovine serum, 10%
Temperature: 37.0°C
Subculturing: Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:4 is recommended
Medium Renewal: Every 2 to 3 days
Remove medium, and rinse with 0.25% trypsin, 0.03% EDTA solution. Remove the solution and add an additional 1 to 2 ml of trypsin-EDTA solution. Allow the flask to sit at room temperature (or at 37C) until the cells detach.
Add fresh culture medium, aspirate and dispense into new culture flasks.
Preservation: Culture medium, 95%; DMSO, 5%
Related Products: Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2001
recommended serum:ATCC 30-2020
References: 39920: Isaacs JT, et al. AT3B-1细胞Establishment and characterization of seven Dunning rat prostatic cancer cell lines and their use in developing methods for predicting metastatic abilities of prostatic cancers. Prostate 9: 261-281, 1986. PubMed: 3774632
39921: Replogle-Schwab TS, et al. Development of doxorubicin resistant rat prostate cancer cell lines. Anticancer Res. 17: 4535-4538, 1997. PubMed: 9494564
