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TD.1细胞

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  • 产品名称:TD.1细胞
  • 产品型号:TD.1
  • 产品展商:HZbscience
  • 产品文档:无相关文档
  • 发布时间:2016-10-08
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简单介绍
TD.1细胞应如何避免细胞污染,细胞污染的种类可分成**、酵母菌、霉菌、病毒和霉浆菌。主要的污染原因为无菌操作技术不当、操作室环境不佳、污染之血清和污染之细胞等。严格之无菌操作技术、清洁的环境、与品质良好之细胞来源和培养基配制是减低污染之*好方法。TD.1细胞何时须更换培养基?视细胞生长密度而定,或遵照细胞株基本数据上之更换时间,按时更换培养基即可。
产品描述

TD.1细胞

细胞形态: **样

**类型: IgG1

生长状态: 悬浮生长

运输方式: 冻存运输

数量: 大量

ATCC Number: CRL-2232™

是否是肿瘤细胞: 0

物种来源: 小鼠

TD.1细胞Designations: TD.1

Depositors: FM Brodsky

Isotype: IgG1

Biosafety Level: 1

Shipped: frozen

Medium & Serum: See Propagation

Growth Properties: suspension

Organism: Mus musculus (B cell); Mus musculus (myeloma) deposited as mouse (B cell); mouse (myeloma)

Morphology: lymphoblast


Source: Cell Type: TD.1细胞hybridoma: B lymphocyte;

Cellular Products: immunoglobulin; monoclonal antibody; against human clathrin heavy chain

Permits/Forms: In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.

Comments: Animals were immunized with the globular N-terminal domain of clathrin heavy chain.

TD.1细胞Spleen cells were fused with Sp2/0-Ag14 myeloma cells.

The antibody is useful for blotting of clathrin heavy chains from a variety of species. It does not react with native clathrin in immunofluorescence or immunoprecipitation assays.

Propagation: ATCC complete growth medium: The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, Catalog No. 30-2001. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 15%.

Subculturing: Medium Renewal: Every 2 to 3 days

Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 0.5 X 10 exp5 to 1 X 10 exp5 cells/ml and maintain between 1 X 10 exp5 and 1 X 10 exp6 cells/ml.

Preservation: Culture medium, 95%; DMSO, 5%

References: 22634: Nathke IS, et al. TD.1细胞Folding and trimerization of clathrin subunits at the triskelion hub. Cell 68: 899-910, 1992. PubMed: 1547490

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