18C2.8.3细胞
数量： 大量
ATCC Number： HB-9571™
运输方式： 冻存运输
生长状态： 悬浮生长
细胞形态： **样
是否是肿瘤细胞： 0
物种来源： 小鼠
Designations: 18C2.8.3
18C2.8.3细胞Depositors: Univ. Georgia Res. Fndn.
Biosafety Level: 1
Shipped: frozen
Medium & Serum: See Propagation
Growth Properties: suspension
Organism: Mus musculus (B cell); Mus musculus (myeloma) deposited as mouse (B cell); mouse (myeloma)
Morphology: lymphoblast
Source: Cell Type: 18C2.8.3细胞hybridoma: B lymphocyte;
Cellular Products: immunoglobulin; monoclonal antibody; against NK cell target ligand on NC-37 cells
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Tumorigenic: Yes
Comments: Animals were immunized with NC-37 (see ATCC CCL-214) cells.
Spleen cells were fused with P3X63Ag8.653 myeloma cells.
18C2.8.3细胞The antibody reacts with NC-37 cells and inhibits NK cell mediated lysis.
The antibody also blocks lysis by catfish nonspecific cytotoxic cells (NCC) which are analogous to mammalian NK cells.
Propagation: ATCC complete growth medium: The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, Catalog No. 30-2001. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Subculturing: Medium Renewal: Every 2 to 3 days
Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 X 10 exp5 cells/ml and maintain between 2 X 10 exp5 and 1 X 10 exp6 cells/ml.
Preservation: Culture medium, 95%; DMSO, 5%
References: 22020: Evans DL. 18C2.8.3细胞Antibodies to natural killer cell and non-specific cytotoxic cell receptor and target cell antigens. US Patent 5,194,593 dated Mar 16 1993
