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1H11细胞

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  • 产品名称:1H11细胞
  • 产品型号:1H11
  • 产品展商:HZbscience
  • 产品文档:无相关文档
  • 发布时间:2018-07-24
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简单介绍
1H11细胞应如何避免细胞污染,细胞污染的种类可分成**、酵母菌、霉菌、病毒和霉浆菌。主要的污染原因为无菌操作技术不当、操作室环境不佳、污染之血清和污染之细胞等。严格之无菌操作技术、清洁的环境、与品质良好之细胞来源和培养基配制是减低污染之*好方法。1H11细胞何时须更换培养基?视细胞生长密度而定,或遵照细胞株基本数据上之更换时间,按时更换培养基即可。
产品描述

1H11细胞

ATCC Number: HB-10611™

生长状态: 悬浮生长

细胞形态: **样

**类型: IgG1

运输方式: 冻存运输

数量: 大量

是否是肿瘤细胞: 0

1H11细胞物种来源: 小鼠

Designations: 1H11

Depositors: Washington Research Foundation

Isotype: IgG1

Biosafety Level: 1

Shipped: frozen

Medium & Serum: See Propagation

Growth Properties: suspension

Organism: Mus musculus (B cell); Mus musculus (myeloma) deposited as mouse (B cell); mouse (myeloma)

Morphology: lymphoblast


Source: 1H11细胞Cell Type: hybridoma: B lymphocyte;

Cellular Products: immunoglobulin; monoclonal antibody; against bone type 1 collagen amino terminal telopeptide

Permits/Forms: In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.

Comments: Animals were immunized with a conjugate of keyhole limpet hemocyanin (KLH) and a telopeptide having a 3-hydroxypyridinium crosslink derived from the amino terminal domain of bone type I collagen.

Spleen cells were fused with mouse myeloma cells.

The antibody can be used to detect and quantitate degradation products of bone type I collagen in body fluids.

Propagation: 1H11细胞ATCC complete growth medium: The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.

Temperature: 37.0°C

Subculturing: Medium Renewal: Every 2 to 3 days

Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 X 10 exp5 cells/ml and maintain between 1 X 10 exp5 and 1 X 10 exp6 cells/ml.

References: 22055: Eyre DR. 1H11细胞Hybridoma cell line producing an antibody to type-I collagen amino-terminal telopeptide. US Patent 5,300,434 dated Apr 5 1994

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