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TC31细胞

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  • 产品名称:TC31细胞
  • 产品型号:TC31
  • 产品展商:HZbscience
  • 产品文档:无相关文档
  • 发布时间:2016-10-08
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简单介绍
TC31细胞应如何避免细胞污染,细胞污染的种类可分成**、酵母菌、霉菌、病毒和霉浆菌。主要的污染原因为无菌操作技术不当、操作室环境不佳、污染之血清和污染之细胞等。严格之无菌操作技术、清洁的环境、与品质良好之细胞来源和培养基配制是减低污染之*好方法。TC31细胞何时须更换培养基?视细胞生长密度而定,或遵照细胞株基本数据上之更换时间,按时更换培养基即可。
产品描述

TC31细胞

运输方式: 冻存运输

是否是肿瘤细胞: 0

物种来源: 小鼠

生长状态: 悬浮生长

数量: 大量

ATCC Number: CRL-2494™

细胞形态: **样

**类型: IgM kappa

TC31细胞Designations: TC31

Depositors: DG Sieckmann

Isotype: IgM kappa

Biosafety Level: 1

Shipped: frozen

Medium & Serum: See Propagation

Growth Properties: suspension

Organism: Mus musculus (B cell); Mus musculus (myeloma) deposited as mouse (B cell); mouse (myeloma)

Morphology: lymphoblast


Source: Cell Type: hybridoma:lymphoblast B lymphocyte; somatic cell hybrid

Permits/Forms: TC31细胞In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.

Restrictions: This cell line was deposited at the ATCC by Donna G. Sieckmann, Naval Medical Research Center. The line is distributed for academic research purposes only. The Naval Medical Research Center releases the line subject to the following: 1.) The line or its products must not be distributed to third parties. Commercial interests are the exclusive property of the Naval Medical Research Center. 2.) Any proposed commercial use of the cell line including any use by a for-profit entity must first be negotiated with the Commanding Officer, Naval Medical Research Center, 503 Robert Grant Avenue, Silver Spring, MD 20910-7500.

Isolation: Isolation date: 1984

Comments: Animals were immunized against exotoxin A of Pseudomonas aeruginosa by infection with strain PA220.

Spleen cells were fused with Sp2/0-Ag14 cells.

The antibody binds specifically for the exotoxin A of Pseudomonas aeruginosa.

TC31细胞It displays specificity for exotoxin A from strain PA103 and strain PAO1 and PAO-PR1, a derivative of PAO1. [40007]

The antibody shows high specificity and reactivity for native and denatured exotoxin A in ELISA assays.

Both TC1 (ATCC CRL-2493) and TC31 (ATCC CRL-2485).ATCC CRL-2494) antibodies neutralize exotoxin A toxicity against CHO cells in an in vitro assay but only TC31 protected mice infected with strain PA220 in an in vivo model. [40007]

A hybridoma producing an anti idiotypic antibody against TC31 is available (

Propagation: ATCC complete growth medium: Iscove's modified Dulbecco's medium with 4 mM L-glutamine adjusted to contain 1.5 g/L sodium bicarbonate, 85%; fetal bovine serum, 15%

Atmosphere: air, 95%; carbon dioxide (CO2), 5%

Temperature: 37.0°C

Subculturing: Protocol: Cultures can be maintained by addition of fresh medium. Alternatively, cultures can be established by centrifugation with subsequent resuspension at 5 X 10(4) viable cells/ml. Do not allow the cell concentration to exceed 5 X 10(5) viable cells/ml.

Interval: Maintain cultures at a cell concentration between 5 X 10(4) and 5 X 10(5) cells/ml.

Medium Renewal: Add fresh medium every 2 to 3 days (depending on cell density)

Preservation: Freeze medium: TC31细胞Complete growth medium supplemented with 5% (v/v) DMSO

Storage temperature: liquid nitrogen vapor phase

Related Products: Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2005

recommended serum:ATCC 30-2020

References: 40007: Galloway DR, et al. Production and characterization of monoclonal antibodies to exotoxin A from Pseudomonas aeruginosa. Infect. Immun. 44: 262-267, 1984. PubMed: 6425217

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