1H3细胞
ATCC Number： HB-284™
是否是肿瘤细胞： 0
物种来源： 小鼠
生长状态： 悬浮生长
数量： 大量
运输方式： 冻存运输
细胞形态： **样
**类型： IgG2a
1H3细胞Designations: 1H3
Depositors: Mo Dailey, JW Shepherd
Isotype: IgG2a
Biosafety Level: 1
Shipped: frozen
Medium & Serum: See Propagation
Growth Properties: suspension
Organism: Mus musculus (B cell); Mus musculus (myeloma) deposited as mouse (B cell); mouse (myeloma)
Morphology: lymphoblast
Source: Cell Type: 1H3细胞hybridoma: B lymphocyte;
Cellular Products: immunoglobulin; monoclonal antibody; against human CD62L
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Comments: Animals were immunized with human peripheral blood leukocytes.
Spleen cells were fused with Sp2/0-Ag14 myeloma cells.
The antibody reacts with a cell surface molecule (CD62L) that mediates the initial adhesion of lymphocytes and neutrophils to endothelial cells.
1H3细胞The antibody can be used in both immunofluorescence and immunoprecipitation assys.
The cells can be adapted to serum free growth in HB101 medium.
Propagation: ATCC complete growth medium: The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, Catalog No. 30-2001. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Subculturing: Medium Renewal: Every 2 to 3 days
Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 X 10 exp5 cells/ml and maintain between 1 X 10 exp5 and 1 X 10 exp6 cells/ml.
Preservation: 1H3细胞Culture medium, 95%; DMSO, 5%
References: 22158: . . FASEB J. 6: 1142, 1992.
