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SW 156细胞

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  • 产品名称:SW 156细胞
  • 产品型号:SW 156
  • 产品展商:HZbscience
  • 产品文档:无相关文档
  • 发布时间:2018-09-05
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简单介绍
SW 156细胞应如何避免细胞污染,细胞污染的种类可分成**、酵母菌、霉菌、病毒和霉浆菌。主要的污染原因为无菌操作技术不当、操作室环境不佳、污染之血清和污染之细胞等。严格之无菌操作技术、清洁的环境、与品质良好之细胞来源和培养基配制是减低污染之*好方法。SW 156细胞何时须更换培养基?视细胞生长密度而定,或遵照细胞株基本数据上之更换时间,按时更换培养基即可。
产品描述

SW 156细胞

器官来源: 肾脏

细胞形态: 上皮样

生长状态: 贴壁生长

运输方式: 冻存运输

数量: 大量

是否是肿瘤细胞: 1

物种来源: 人

ATCC Number: CRL-2175™

相关**: 其他**

SW 156细胞年限: 75 years

Designations: SW 156 [SW-156, SW156]

Depositors: W McCombs

Biosafety Level: 1

Shipped: frozen

Medium & Serum: See Propagation

Growth Properties: adherent

Organism: Homo sapiens

Morphology: epithelial


Source: Organ: kidney

Disease: hypernephroma

Permits/Forms: SW 156细胞In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.

Restrictions: These cells are distributed for research purposes only. The Scott and White Clinic releases the line subject to the following: 1) The cells or products derived from them must not be sold or used for commercial purposes. Nor can the cells be distributed to third parties for purpose of sale, or producing for sale, cells or their products. Commercial interests are the exclusive property of the Scott and White Clinic. 2) Any proposed commercial use of these cells or products produced by them must first be negotiated with the Scott and White Clinic, 2401 S. 31 Street, Temple, Texas 76508. Telephone (817) 774-2432

Antigen Expression: Blood Type O; Rh +

Age: 75 years

Gender: male

Ethnicity: Caucasian

Comments: The SW 156 line was established in 1972 by A. Leibovitz from a hypernephroma.

SW 156细胞Propagation: ATCC complete growth medium: Leibovitz's L-15 medium with 2mM L-glutamine, 90%; fetal bovine serum, 10%

Subculturing: Subcultivation Ratio: A subcultivation ratio of 1:4 to 1:6 is recommended

Medium Renewal: 2 to 3 times per week

Remove spent medium, add fresh 0.25% trypsin, 0.03% EDTA solution, rinse and remove trypsin.

Add fresh trypsin solution (1 to 2 ml), and let the culture sit at room temperature (or at 37C) until the cells detach.

Add fresh medium, aspirate and dispense into new flasks.

Preservation: Culture medium, 95%; DMSO, 5%

References: 22536: Fogh J, et al. SW 156细胞Absence of HeLa cell contamination in 169 cell lines derived from human tumors. J. Natl. Cancer Inst. 58: 209-214, 1977. PubMed: 833871

24381: Fogh J. Cultivation, characterization, and identification of human tumor cells with emphasis on kidney, testis, and bladder tumors. Natl. Cancer Inst. Monogr. 49: 5-9, 1978. PubMed: 571047

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