SW1463细胞
年限： Dukes' type C
ATCC Number： CCL-234™
相关**： 大肠癌
生长状态： 贴壁生长
数量： 大量
器官来源： 直肠
细胞形态： 上皮样
运输方式： 冻存运输
是否是肿瘤细胞： 1
物种来源： 人
SW1463细胞Designations: SW1463 [SW 1463, SW-1463]
Depositors: A Leibovitz
Biosafety Level: 1
Shipped: frozen
Medium & Serum: See Propagation
Growth Properties: adherent
Organism: Homo sapiens
Morphology: epithelial
Source: Organ: rectum
Tumor Stage: Dukes' type C
Disease: colorectal adenocarcinoma
Cellular Products: carcinoembryonic antigen (CEA) 223 ng/10 exp6 cells/10 days; keratin
Permits/Forms: SW1463细胞In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
Tumorigenic: Yes
Antigen Expression: blood type A; Rh +
DNA Profile (STR): Amelogenin: X
CSF1PO: 11,12
D13S317: 12,13
D16S539: 11
D5S818: 13,14
D7S820: 9
THO1: 6,7
TPOX: 8,11
vWA: 16
Isoenzymes: ES-D, 1
G6PD, B
PEP-D, 1
SW1463细胞PGD, A
PGM1, 1
PGM3, 1-2
Age: 66 years
Gender: female
Ethnicity: Caucasian
Comments: The line was derived from a grade II mucous producing and grade III solid tumor.
CSAp negative (CSAp-).
The cells are positive for keratin by immunoperoxidase staining.
Propagation: ATCC complete growth medium: The base medium for this cell line is ATCC-formulated Leibovitz's L-15 Medium, Catalog No. 30-2008. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Atmosphere: air, 100%
Subculturing: Subcultivation Ratio: A subcultivation ratio of 1:3 to 1:8 is recommended
Medium Renewal: 1 to 2 times per week
Remove medium, add fresh 0.25% trypsin, 0.02% EDTA for 10 to 20 minutes at 37C. Add fresh medium, disperse cells and centrifuge to pellet the cells.
Resuspend in fresh medium and dispense into new flasks.
Subculture just prior to confluency (about 10 to 12 days).
References: 23025: Leibovitz A, et al. SW1463细胞Classification of human colorectal adenocarcinoma cell lines. Cancer Res. 36: 4562-4569, 1976. PubMed: 1000501
