RL95-2细胞
细胞形态： 上皮样
运输方式： 冻存运输
组织来源： endometrium
ATCC Number： CRL-1671™
相关**： 肿瘤
器官来源： **
是否是肿瘤细胞： 1
物种来源： 人
生长状态： 贴壁生长
年限： 65 years
数量： 大量
RL95-2细胞Designations: RL95-2
Depositors: DL Way
Biosafety Level: 1
Shipped: frozen
Medium & Serum: See Propagation
Growth Properties: adherent
Organism: Homo sapiens
Morphology: epithelial
Source: Organ: uterus
Tissue: endometrium
Disease: carcinoma
Permits/Forms: RL95-2细胞In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
Receptors: estrogen receptor, positive
DNA Profile (STR): Amelogenin: X
CSF1PO: 10,11
D13S317: 8,12
D16S539: 11,13
D5S818: 10,11
D7S820: 10
THO1: 9,9.3
TPOX: 8
vWA: 16,20
Age: 65 years
Gender: female
Ethnicity: RL95-2细胞Caucasian
Comments: The cells possess alpha keratin, well defined junctional complexes, tonofilaments and surface microvilli.
Propagation: ATCC complete growth medium: The base medium for this cell line is ATCC-formulated DMEM:F12 Medium Catalog No. 30-2006. To make the complete growth medium, add the following components to the base medium:
0.005 mg/ml insulin
fetal bovine serum to a final concentration of 10%
Temperature: 37.0°C
Subculturing: Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:5 is recommended
Medium Renewal: Every 2 to 3 days
Remove medium, and rinse with 0.25% trypsin, 0.03% EDTA solution. Remove the solution and add an additional 1 to 2 ml of trypsin-EDTA solution. Allow the flask to sit at room temperature (or at 37C) until the cells detach.
Add fresh culture medium, aspirate and dispense into new culture flasks.
Preservation: culture medium 95%; DMSO, 5%
Related Products: Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2006
recommended serum:ATCC 30-2020
References: 2151: RL95-2细胞Way DL, et al. Characterization of a new human endometrial carcinoma (RL95-2) established in tissue culture. In Vitro 19: 147-158, 1983. PubMed: 6339371
22666: Cohen LA. Isolation and characterization of a serially cultivated, neoplastic, epithelial cell line from the N-nitrosomethylurea induced rat mammary adenocarcinoma. In Vitro 18: 565-575, 1982. PubMed: 7118137
29988: Hendricks DT, et al. FHIT gene expression in human ovarian, endometrial, and cervical cancer cell lines. Cancer Res. 57: 2112-2115, 1997. PubMed: 9187105
32565: Yang N, et al. Estrogen-related receptor, hERR1, modulates estrogen receptor-mediated response of human lactoferrin gene promoter. J. Biol. Chem. 271: 5795-5804, 1996. PubMed: 8621448
