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MDA-MB-361细胞

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  • 产品名称:MDA-MB-361细胞
  • 产品型号:MDA-MB-361
  • 产品展商:HZbscience
  • 产品文档:无相关文档
  • 发布时间:2018-09-04
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简单介绍
MDA-MB-361细胞应如何避免细胞污染,细胞污染的种类可分成**、酵母菌、霉菌、病毒和霉浆菌。主要的污染原因为无菌操作技术不当、操作室环境不佳、污染之血清和污染之细胞等。严格之无菌操作技术、清洁的环境、与品质良好之细胞来源和培养基配制是减低污染之*好方法。MDA-MB-361细胞何时须更换培养基?视细胞生长密度而定,或遵照细胞株基本数据上之更换时间,按时更换培养基即可。
产品描述

MDA-MB-361细胞

生长状态: 贴壁生长

年限: 40 years

ATCC Number: HTB-27™

相关**: 腺癌

数量: 大量

器官来源: **

运输方式: 冻存运输

细胞形态: 上皮样

是否是肿瘤细胞: 1

物种来源: 人

Designations: MDA-MB-361

MDA-MB-361细胞Depositors: R Cailleau

Biosafety Level: 1

Shipped: frozen

Medium & Serum: See Propagation

Growth Properties: loosely adherent

Organism: Homo sapiens

Morphology: epithelial


Source: Organ: mammary gland; breast

Disease: adenocarcinoma

Derived from metastatic site: brain

Permits/Forms: In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. MDA-MB-361细胞Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.

Oncogene: wnt7h +

DNA Profile (STR): Amelogenin: X

CSF1PO: 12

D13S317: 11

D16S539: 11,12

D5S818: 10,11

D7S820: 9,12

THO1: 9.3

TPOX: 8,11

vWA: 17

Cytogenetic Analysis: modal number. = 56; range = 54 to 61.

The cell line is aneuploid human female, with chromosome counts in the hyperdiploid range. Normal chromosomes N11 and N17 are absent, chromosomes N1, N20, and N21 are weakly represented, and chromosomes N2, N8, N9, and N15 are single. The remainder of chromosomes are often paired. MDA-MB-361细胞Eighteen marker chromosomes are found, of which 10 are consistently present. Some of these markers are found to be quite comparable to those described by K.L. Satya-Prakash, et al., in their report on this cell line.

Isoenzymes: AK-1, 1

ES-D, 1

G6PD, B

GLO-I, 2

Me-2, 1

PGM1, 1

PGM3, 1-2

Age: 40 years

Gender: female

Ethnicity: Caucasian

Comments: This line differs from others of the series (ATCC HTB-23 to 26) in karyology and in that it was isolated from a brain metastasis.

Propagation: ATCC complete growth medium: The base medium for this cell line is ATCC-formulated Leibovitz's L-15 Medium, Catalog No. 30-2008. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 20%.

Temperature: 37.0°C

Atmosphere: air, 100%

Subculturing: Protocol:

Remove and discard culture medium.

Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum which contains trypsin inhibitor.

Add 2.0 to 3.0 ml of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).

Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37�C to facilitate dispersal.

Add 6.0 to 8.0 ml of complete growth medium and aspirate cells by gently pipetting.

Add appropriate aliquots of the cell suspension to new culture vessels.

Incubate cultures at 37�C.


Subcultivation Ratio: MDA-MB-361细胞A subcultivation ratio of 1:2 to 1:6 is recommended

Medium Renewal: 2 to 3 times per week

Preservation: Freeze medium: Complete growth medium, 95%; DMSO, 5%

Storage temperature: liquid nitrogen vapor phase

Related Products: Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2008

recommended serum:ATCC 30-2020

References: 1206: Brinkley BR, et al. Variations in cell form and cytoskeleton in human breast carcinoma cells in vitro. Cancer Res. 40: 3118-3129, 1980. PubMed: 7000337

22182: Cruciger Q, et al. Morphological, biochemical and chromosomal characterization of breast tumor lines from pleural effusions. In Vitro 12: 331, 1976.

22429: Siciliano MJ, et al. Mutually exclusive genetic signatures of human breast tumor cell lines with a common chromosomal marker. Cancer Res. 39: 919-922, 1979. PubMed: 427779

22532: Cailleau R, et al. Breast tumor cell lines from pleural effusions. J. Natl. Cancer Inst. 53: 661-674, 1974. PubMed: 4412247

22536: Fogh J, et al. Absence of HeLa cell contamination in 169 cell lines derived from human tumors. J. Natl. Cancer Inst. 58: 209-214, 1977. PubMed: 833871

22656: Cailleau R, et al. Long-term human breast carcinoma cell lines of metastatic origin: preliminary characterization. In Vitro 14: 911-915, 1978. PubMed: 730202

23093: Faust JB, Meeker TC. Amplification and expression of the bcl-1 gene in human solid tumor cell lines. Cancer Res. 52: 2460-2463, 1992. PubMed: 1568216

23113: Huguet EL, et al. Differential expression of human Wnt genes 2, 3, 4, and 7B in human breast cell lines and normal and disease states of human breast tissue. Cancer Res. 54: 2615-2621, 1994. PubMed: 8168088

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