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A2058细胞

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  • 产品名称:A2058细胞
  • 产品型号:A2058
  • 产品展商:HZbscience
  • 产品文档:无相关文档
  • 发布时间:2018-07-20
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简单介绍
A2058细胞应如何避免细胞污染,细胞污染的种类可分成**、酵母菌、霉菌、病毒和霉浆菌。主要的污染原因为无菌操作技术不当、操作室环境不佳、污染之血清和污染之细胞等。严格之无菌操作技术、清洁的环境、与品质良好之细胞来源和培养基配制是减低污染之*好方法。A2058细胞何时须更换培养基?视细胞生长密度而定,或遵照细胞株基本数据上之更换时间,按时更换培养基即可。
产品描述

A2058细胞

ATCC Number: CRL-11147™

相关**: 黑色素瘤

是否是肿瘤细胞: 1

物种来源: 人

数量: 大量

器官来源: 皮肤

细胞形态: 上皮样

运输方式: 冻存运输

年限: 43 years *****

生长状态: 贴壁生长

Designations: A2058

A2058细胞Depositors: W Stetler-Stevenson

Biosafety Level: 1

Shipped: frozen

Medium & Serum: See Propagation

Growth Properties: adherent

Organism: Homo sapiens

Morphology: epithelial


Source: Organ: skin

Disease: melanoma

Derived from metastatic site: lymph node

Permits/Forms: In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. A2058细胞Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.

Applications: transfection host

Receptors: nerve growth factor (NGF), expressed

laminin, expressed

Tumorigenic: Yes

DNA Profile (STR): Amelogenin: X,Y

CSF1PO: 10,11

D13S317: 13,14

D16S539: 9,13

D5S818: 9,12

D7S820: 11

THO1: 7,9

TPOX: 8

vWA: 14,18

Age: 43 years *****

Gender: male

Ethnicity: A2058细胞Caucasian

Comments: This cell line is highly invasive and provides a source of cellular invasion associated proteins (such as the 72000 dalton type IV collagenase.

Tissue inhibitor of metalloproteinase-2 [TIMP-2], autocrine motility factor and the 67000 dalton laminin receptor.

Propagation: ATCC complete growth medium: The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.

Atmosphere: air, 95%; carbon dioxide (CO2), 5%

Temperature: 37.0°C

Subculturing: Protocol:

Remove and discard culture medium.

Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.

Add 2.0 to 3.0 ml of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).

Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37�C to facilitate dispersal.

Add 6.0 to 8.0 ml of complete growth medium and aspirate cells by gently pipetting.

Add appropriate aliquots of the cell suspension to new culture vessels.

Incubate cultures at 37�C.


Subcultivation Ratio: A2058细胞A subcultivation ratio of 1:6 to 1:12 is recommended

Medium Renewal: Every 2 to 3 days

Preservation: Freeze medium: Complete growth medium supplemented with 5% (v/v) DMSO

Storage temperature: liquid nitrogen vapor phase

Related Products: Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2002

recommended serum:ATCC 30-2020

References: 22590: Fabricant RN, et al. Nerve growth factor receptors on human melanoma cells in culture. Proc. Natl. Acad. Sci. USA 74: 565-569, 1977. PubMed: 265522

23263: Sherwin SA, et al. Human melanoma cells have both nerve growth factor and nerve growth factor-specific receptors on their cell surfaces. Proc. Natl. Acad. Sci. USA 76: 1288-1292, 1979. PubMed: 375235

23269: Todaro GJ, et al. Transforming growth factors produced by certain human tumor cells: polypeptides that interact with epidermal growth factor receptors. Proc. Natl. Acad. Sci. USA 77: 5258-5262, 1980. PubMed: 6254071

23404: Stetler-Stevenson WG, et al. The activation of human type IV collagenase proenzyme. Sequence identification of the major conversion product following organomercurial activation. J. Biol. Chem. 264: 1353-1356, 1989. PubMed: 2536363

23549: Stetler-Stevenson WG, et al. Tissue inhibitor of metalloproteinase (TIMP-2). A new member of the metalloproteinase inhibitor family. J. Biol. Chem. 264: 17374-17378, 1989. PubMed: 2793861

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