A2058细胞
ATCC Number： CRL-11147™
相关**： 黑色素瘤
是否是肿瘤细胞： 1
物种来源： 人
数量： 大量
器官来源： 皮肤
细胞形态： 上皮样
运输方式： 冻存运输
年限： 43 years *****
生长状态： 贴壁生长
Designations: A2058
A2058细胞Depositors: W Stetler-Stevenson
Biosafety Level: 1
Shipped: frozen
Medium & Serum: See Propagation
Growth Properties: adherent
Organism: Homo sapiens
Morphology: epithelial
Source: Organ: skin
Disease: melanoma
Derived from metastatic site: lymph node
Permits/Forms: In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. A2058细胞Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
Applications: transfection host
Receptors: nerve growth factor (NGF), expressed
laminin, expressed
Tumorigenic: Yes
DNA Profile (STR): Amelogenin: X,Y
CSF1PO: 10,11
D13S317: 13,14
D16S539: 9,13
D5S818: 9,12
D7S820: 11
THO1: 7,9
TPOX: 8
vWA: 14,18
Age: 43 years *****
Gender: male
Ethnicity: A2058细胞Caucasian
Comments: This cell line is highly invasive and provides a source of cellular invasion associated proteins (such as the 72000 dalton type IV collagenase.
Tissue inhibitor of metalloproteinase-2 [TIMP-2], autocrine motility factor and the 67000 dalton laminin receptor.
Propagation: ATCC complete growth medium: The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Atmosphere: air, 95%; carbon dioxide (CO2), 5%
Temperature: 37.0°C
Subculturing: Protocol:
Remove and discard culture medium.
Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.
Add 2.0 to 3.0 ml of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).
Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37�C to facilitate dispersal.
Add 6.0 to 8.0 ml of complete growth medium and aspirate cells by gently pipetting.
Add appropriate aliquots of the cell suspension to new culture vessels.
Incubate cultures at 37�C.
Subcultivation Ratio: A2058细胞A subcultivation ratio of 1:6 to 1:12 is recommended
Medium Renewal: Every 2 to 3 days
Preservation: Freeze medium: Complete growth medium supplemented with 5% (v/v) DMSO
Storage temperature: liquid nitrogen vapor phase
Related Products: Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2002
recommended serum:ATCC 30-2020
References: 22590: Fabricant RN, et al. Nerve growth factor receptors on human melanoma cells in culture. Proc. Natl. Acad. Sci. USA 74: 565-569, 1977. PubMed: 265522
23263: Sherwin SA, et al. Human melanoma cells have both nerve growth factor and nerve growth factor-specific receptors on their cell surfaces. Proc. Natl. Acad. Sci. USA 76: 1288-1292, 1979. PubMed: 375235
23269: Todaro GJ, et al. Transforming growth factors produced by certain human tumor cells: polypeptides that interact with epidermal growth factor receptors. Proc. Natl. Acad. Sci. USA 77: 5258-5262, 1980. PubMed: 6254071
23404: Stetler-Stevenson WG, et al. The activation of human type IV collagenase proenzyme. Sequence identification of the major conversion product following organomercurial activation. J. Biol. Chem. 264: 1353-1356, 1989. PubMed: 2536363
23549: Stetler-Stevenson WG, et al. Tissue inhibitor of metalloproteinase (TIMP-2). A new member of the metalloproteinase inhibitor family. J. Biol. Chem. 264: 17374-17378, 1989. PubMed: 2793861
