MB 157细胞
生长状态： 贴壁生长
ATCC Number： CRL-7721™
相关**： 肿瘤
年限： 48 years *****
器官来源： **
数量： 大量
组织来源： pleural effusion
运输方式： 冻存运输
是否是肿瘤细胞： 1
物种来源： 人
细胞形态： 上皮样
MB 157细胞Designations: MB 157
Biosafety Level: 1
Shipped: frozen
Medium & Serum: See Propagation
Growth Properties: adherent
Organism: Homo sapiens
Morphology: epithelial
Source: Organ: mammary gland; breast
Tissue: pleural effusion
Disease: carcinoma
Derived from metastatic site: pleural effusion
Permits/Forms: MB 157细胞In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
Tumorigenic: Yes
DNA Profile (STR): Amelogenin: X
CSF1PO: 10
D13S317: 11
D16S539: 11
D5S818: 12
D7S820: 10,11
THO1: 7,8
TPOX: 9,11
vWA: 15
Cytogenetic Analysis: modal number = 62; range = 59 to 64
Isoenzymes: G6PD, B
Age: 48 years *****
MB 157细胞Gender: female
Ethnicity: Black
Comments: Part of the NBL Cell Line Collection. This cell line is neither produced nor fully characterized by ATCC . We do not guarantee that it will maintain a specific morphology, purity, or any other property upon passage.
Please see the NBL Repository description.
Propagation: ATCC complete growth medium: The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Atmosphere: air, 95%; carbon dioxide (CO2), 5%
Temperature: 37.0°C
Subculturing: Protocol: Remove medium, and rinse with 0.25% trypsin, 0.03% EDTA solution. Remove the solution and add an additional 1 to 2 ml of trypsin-EDTA solution. Allow the flask to sit at room temperature (or at 37C) until the cells detach.Add fresh culture medium, aspirate and dispense into new culture flasks.
Subcultivation Ratio: 1:2 to 1:3
Medium Renewal: every 2 to 3 days
Preservation: MB 157细胞Freeze medium: Complete growth medium, 95%; DMSO, 5%
Storage temperature: liquid nitrogen vapor phase
References: 22398: Young RK, et al. Establishment of epithelial cell line MDA-MB-157 from metastatic pleural effusion of human breast carcinoma. In Vitro 9: 239-245, 1974. PubMed: 4471183
22532: Cailleau R, et al. Breast tumor cell lines from pleural effusions. J. Natl. Cancer Inst. 53: 661-674, 1974. PubMed: 4412247
