MIA PaCa-2细胞
生长状态: 贴壁生长
器官来源: 胰腺
ATCC Number: CRL-1420™
相关**: 肿瘤
年限: 65 years
数量: 大量
是否是肿瘤细胞: 1
物种来源: 人
运输方式: 冻存运输
细胞形态: 其他
Designations: MIA PaCa-2
Depositors: A Yunis
MIA PaCa-2细胞Biosafety Level: 1
Shipped: frozen
Medium & Serum: See Propagation
Growth Properties: adherent, single cells and loosely attached clusters
Organism: Homo sapiens
Morphology: attached epithelial with floating rounded cells
Source: Organ: pancreas
Disease: carcinoma
Cellular Products: human colony stimulating factor, subclass I (CSF-I); plasminogen activator
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Isolation: Isolation date: 1975
Applications: MIA PaCa-2细胞The MIA PaCa-2 cell line was established by A. Yunis, et al.
Hybridomas have been made against the CSF-I produced by this line ATCC HB-8207 (F1A3-23) and ATCC HB-8208 (F18 AF1).
The established cell line reportedly has a doubling time of about 40 hours and a colony-forming efficiency in soft agar of approximately 19%.
The line is sensitive to asparaginase.
DNA Profile (STR): Amelogenin: X
CSF1PO: 10
D13S317: 12,13
D16S539: 10,13
D5S818: 12,13
D7S820: 12,13
THO1: 9,10
TPOX: 9
vWA: 15
Cytogenetic Analysis: This is a hypotriploid human cell line. The modal chromosome number is 61. Sixteen to 20 marker chromosomes are commonly found in a cell. MIA PaCa-2细胞A few normal chromosomes are absent.
Isoenzymes: G6PD, B
Age: 65 years
Gender: male
Ethnicity: Caucasian
Comments: The MIA PaCa-2 cell line was established by A. Yunis, et al. in 1975 from tumor tissue of the pancreas obtained from a 65-year-old Caucasian male.
The established cell line reportedly has a doubling time of about 40 hours and a colony-forming efficiency in soft agar of approximately 19%.
Hybridomas have been made against the CSF-I produced by this line ATCC HB-8207 (F1A3-23) and ATCC HB-8208 (F18 AF1).
The line is sensitive to asparaginase.
Propagation: ATCC complete growth medium: The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%; horse serum to a final concentration of 2.5%.
Temperature: 37.0°C
Subculturing: Protocol: Remove medium, and rinse with 0.25% trypsin, 0.53 mM EDTA solution. Remove the solution and add an additional 1 to 2 ml of trypsin-EDTA solution. Allow the flask to sit at room temperature (or at 37C) until the cells detach. Add fresh culture medium, aspirate and dispense into new culture flasks.
Subcultivation Ratio: MIA PaCa-2细胞A subcultivation ratio of 1:3 to 1:8 is recommended
Medium Renewal: 2 to 3 times per week
Preservation: Freeze medium: Complete growth medium 95%; DMSO, 5%
Storage temperature: liquid nitrogen vapor phase
Related Products: Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2002
recommended serum:ATCC 30-2020
recommended serum:ATCC 30-2040
References: 1057: Wu M, et al. Purification and characterization of a plasminogen activator secreted by cultured human pancreatic carcinoma cells. Biochemistry 16: 1908-1913, 1977. PubMed: 15590
22291: Fountzilas G, et al. Comparative effects of selected drug combinations on the growth of a human pancreatic carcinoma cell line (MIA PaCa-2). J. Natl. Cancer Inst. 76: 37-43, 1986. PubMed: 3455740
49623: Robertson JF, et al. Effect of gastrointestinal hormones and synthetic analogues on the growth of pancreatic cancer. Int. J. Cancer 63: 69-75, 1995. PubMed: 7558455
58034: Yunis AA, et al. MIA PaCa-2细胞Human pancreatic carcinoma (MIA PaCa-2) in continuous culture: sensitivity to asparaginase. Int. J. Cancer 19: 128-135, 1977. PubMed: 832918