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MDA-MB-436细胞

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  • 产品名称:MDA-MB-436细胞
  • 产品型号:MDA-MB-436
  • 产品展商:HZbscience
  • 产品文档:无相关文档
  • 发布时间:2016-10-09
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简单介绍
MDA-MB-436细胞应如何避免细胞污染,细胞污染的种类可分成**、酵母菌、霉菌、病毒和霉浆菌。主要的污染原因为无菌操作技术不当、操作室环境不佳、污染之血清和污染之细胞等。严格之无菌操作技术、清洁的环境、与品质良好之细胞来源和培养基配制是减低污染之*好方法。MDA-MB-436细胞何时须更换培养基?视细胞生长密度而定,或遵照细胞株基本数据上之更换时间,按时更换培养基即可。
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MDA-MB-436细胞

生长状态: 贴壁生长

数量: 大量

ATCC Number: HTB-130™

相关**: 腺癌

细胞形态: 其他

年限: 43 years

器官来源: **

是否是肿瘤细胞: 1

物种来源: 人

运输方式: 冻存运输

Designations: MDA-MB-436

MDA-MB-436细胞Depositors: R Cailleau

Biosafety Level: 1

Shipped: frozen

Medium & Serum: See Propagation

Growth Properties: adherent

Organism: Homo sapiens

Morphology: pleomorphic with multinucleated component cells


Source: Organ: mammary gland; breast

Disease: adenocarcinoma

Derived from metastatic site: pleural effusion

Cellular Products: tubulin; actin

Permits/Forms: In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.

Tumorigenic: No

DNA Profile (STR): MDA-MB-436细胞Amelogenin: X

CSF1PO: 12

D13S317: 10

D16S539: 9,11

D5S818: 13

D7S820: 10

THO1: 9.3

TPOX: 8

vWA: 14,20

Cytogenetic Analysis: modal number = 45

Isoenzymes: AK-1, 1

ES-D, 1

G6PD, B

GLO-I, 2

Me-2, 1

PGM1, 1

PGM3, 1

Age: 43 years

Gender: female

Ethnicity: MDA-MB-436细胞Caucasian

Comments: The line is pleomorphic and most cells react intensely with anti tubulin antibody as demonstrated by indirect immunofluorescence staining.

Propagation: ATCC complete growth medium: Leibovitz's L-15 medium with 10 mcg/ml insulin, 16 mcg/ml glutathione, 90%; fetal bovine serum, 10%

Temperature: 37.0°C

Atmosphere: air, 100%

Subculturing: Subcultivation Ratio: A subcultivation ratio of 1:2 is recommended

Medium Renewal: 2 to 3 times per week

Subcultures are prepared by scraping. Remove spent medium, add 3 to 5 ml of fresh medium, dislodge cells from the floor of the flask, aspirate and dispense into new flasks. Subculture every 6 to 8 days.

Preservation: Culture medium, 95%; DMSO, 5%

References: 1206: Brinkley BR, et al. Variations in cell form and cytoskeleton in human breast carcinoma cells in vitro. Cancer Res. 40: 3118-3129, 1980. PubMed: 7000337

22429: Siciliano MJ, et al. Mutually exclusive genetic signatures of human breast tumor cell lines with a common chromosomal marker. Cancer Res. 39: 919-922, 1979. PubMed: 427779

22656: Cailleau R, et al. MDA-MB-436细胞Long-term human breast carcinoma cell lines of metastatic origin: preliminary characterization. In Vitro 14: 911-915, 1978. PubMed: 730202

23079: Lan MS, et al. Polypeptide core of a human pancreatic tumor mucin antigen. Cancer Res. 50: 2997-3001, 1990. PubMed: 2334903

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