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CCF-STTG1细胞

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  • 产品名称:CCF-STTG1细胞
  • 产品型号:CCF-STTG1
  • 产品展商:HZbscience
  • 产品文档:无相关文档
  • 发布时间:2018-09-12
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简单介绍
CCF-STTG1细胞应如何避免细胞污染,细胞污染的种类可分成**、酵母菌、霉菌、病毒和霉浆菌。主要的污染原因为无菌操作技术不当、操作室环境不佳、污染之血清和污染之细胞等。严格之无菌操作技术、清洁的环境、与品质良好之细胞来源和培养基配制是减低污染之*好方法。CCF-STTG1细胞何时须更换培养基?视细胞生长密度而定,或遵照细胞株基本数据上之更换时间,按时更换培养基即可。
产品描述

CCF-STTG1细胞


细胞形态: 其他

运输方式: 冻存运输

数量: 大量

年限: grade IV

器官来源: 大脑

生长状态: 贴壁生长

是否是肿瘤细胞: 1

物种来源: 人

ATCC Number: CRL-1718™

相关**: 其他**

CCF-STTG1细胞Designations: CCF-STTG1

Depositors: BP Barna

Biosafety Level: 1

Shipped: frozen

Medium & Serum: See Propagation

Growth Properties: adherent

Organism: Homo sapiens

Morphology: astrocytic


Source: Organ: brain

Tumor Stage: grade IV

Disease: astrocytoma

Permits/Forms: In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. CCF-STTG1细胞Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.

Antigen Expression: HLA-DR; Homo sapiens, expressed

DNA Profile (STR): Amelogenin: X

CSF1PO: 12

D13S317: 11,13

D16S539: 11,12

D5S818: 12,13

D7S820: 10,11

THO1: 7,8

TPOX: 8,11

vWA: 17

Cytogenetic Analysis: This human cell line contained large numbers of tetra-, hexa-, and higher-ploid cells (2s populations). The modal (s) cell population which occurred in 32% of cells had the pseudodiploid karyotype, 46,XX,-3,-18,del(7) (q21.12;q22.3), ?t(3q?18q). The rate of 2s cells was 42%. CCF-STTG1细胞Two marker chromosomes, del(7) (q21.12;q22.3) and ?t(3q?18q), were present in all s metaphases, but ?t(3q?18q) was absent in 2s cells. DMs were found in some s cells whereas they were seen in the majority of 2s cells. HSR chromosomes were not found. In s metaphases, both N3 and N18 had only single copy, and the X chromosome was paired.

Age: 68 years

Gender: female

Ethnicity: Caucasian

Comments: The CCF-STTG1 cell line was established from a specimen of Grade IV astrocytoma from a 68-year-old Caucasian female by mincing and culturing in RPMI 1640 with 10% FBS.

The cells exhibit strong perinuclear acid phosphatase activity and glial fibrillary acidic protein is present in 70% to 80% of the cells.

HLA DR is expressed on 20% to 30% of the cells after 48 hours in culture.

Propagation: ATCC complete growth medium: The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, Catalog No. 30-2001. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.

Atmosphere: air, 95%; carbon dioxide (CO2), 5%

Temperature: 37.0°C

Subculturing: Protocol:

Remove and discard culture medium.

Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.

Add 2.0 to 3.0 ml of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).

Note:CCF-STTG1细胞 To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37�C to facilitate dispersal.

Add 6.0 to 8.0 ml of complete growth medium and aspirate cells by gently pipetting.

Add appropriate aliquots of the cell suspension to new culture vessels.

Incubate cultures at 37�C.


Subcultivation Ratio: A subcultivation ratio of 1:4 to 1:8 is recommended

Medium Renewal: 3 times per week

Preservation: Freeze medium: Complete growth medium supplemented with 5% (v/v) DMSO

Storage temperature: liquid nitrogen vapor phase

Related Products: Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2001

recommended serum:ATCC 30-2020

References: 21868: . Experimental allergic encephalitis. New York: Liss; 1984.

22187: . . In Vitro 19: 275, 1983.

22223: . . Fed. Proc. 43: 781, 1984.

22881: Barna BP, et al. CCF-STTG1细胞Enhanced DNA synthesis of human glial cells exposed to human leukocyte products. J. Neuroimmunol. 10: 151-158, 1985. PubMed: 3877740


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