SCaBER细胞
运输方式： 冻存运输
生长状态： 贴壁生长
ATCC Number： HTB-3™
相关**： 鳞状细胞癌
细胞形态： 上皮样
年限： 58 years
数量： 大量
器官来源： 膀胱
是否是肿瘤细胞： 1
物种来源： 人
Designations: SCaBER
Depositors: C O'Toole
SCaBER细胞Biosafety Level: 1
Shipped: frozen
Medium & Serum: See Propagation
Growth Properties: adherent
Organism: Homo sapiens
Morphology: epithelial
Source: Organ: urinary bladder
Disease: squamous cell carcinoma
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Tumorigenic: Yes
DNA Profile (STR): Amelogenin: X,Y
CSF1PO: 11,13
D13S317: 11,12
D16S539: 11,12
D5S818: 12,13
SCaBER细胞D7S820: 8,10
THO1: 7,9
TPOX: 9,11
vWA: 16
Cytogenetic Analysis: 2n = 46. The stemline chromosome number is hypotriploid with the 2S component occurring at 9.2%., Eleven markers [t(1;?) del (1)(p13), t(3;?), i(4q), t(8;?), t(9;?), M6, t(1;11), t(21;22), M13, and t(20;?)] and 1 to 3 small chromosome fragments were common to most S metaphases., At least one Y chromosome was always detectable. Number 3 was nullisomic. Neither homogenously staining regions (HSR) nor double minutes (DM) were seen.
Isoenzymes: AK-1, 1
ES-D, 1
G6PD, A
GLO-I, 1-2
Me-2, 2
PGM1, 1-2
PGM3, 1-2
Age: 58 years
Gender: male
Ethnicity: Black
Comments: Contains the unusual type A isoenzyme of glucose-6-phosphate dehydrogenase.
Propagation: SCaBER细胞ATCC complete growth medium: Minimum essential medium (Eagle) with 2 mM L-glutamine and Earle's BSS adjusted to contain 1.5 g/L sodium bicarbonate, 0.1 mM non-essential amino acids, and 1.0 mM sodium pyruvate, 90%; fetal bovine serum, 10%
Atmosphere: air, 95%; carbon dioxide (CO2), 5%
Temperature: 37.0°C
Subculturing: Subcultivation Ratio: A subcultivation ratio of 1:3 to 1:6 is recommended
Medium Renewal: 2 to 3 times per week
Remove medium, rinse with fresh 0.25% trypsin, 0.02% EDTA solution, remove trypsin and let the culture sit at room temperature (or at 37C) until the cells detach (about 10 minutes.
Add fresh medium, aspirate and dispense into new flasks.
Subculture every 6 to 8 days.
Preservation: Culture medium, 95%; DMSO, 5%
References: 21849: O'Toole CHuman bladder cancer lines: HLA Class I and Class II antigen expression and susceptibility to cytostatic and cytotoxic effects in vitroIn: O'Toole CIn vitro models for cancer researchvol. IVBoca Raton, FLCRC Presspp. 103-125.
22852: O'Toole C, et al. SCaBER细胞A cell line (SCABER) derived from squamous cell carcinoma of the human urinary bladder. Int. J. Cancer 17: 707-714, 1976. PubMed: 947851
24381: Fogh J. Cultivation, characterization, and identification of human tumor cells with emphasis on kidney, testis, and bladder tumors. Natl. Cancer Inst. Monogr. 49: 5-9, 1978. PubMed: 571047
