SW 684细胞
年限： 68 years
运输方式： 冻存运输
生长状态： 贴壁生长
是否是肿瘤细胞： 1
物种来源： 人
组织来源： connective tissue
数量： 大量
ATCC Number： HTB-91™
相关**： 纤维肉瘤
细胞形态： 成纤维样
Designations: SW 684 [SW-684, SW684]
Depositors: A Leibovitz
SW 684细胞Biosafety Level: 1
Shipped: frozen
Medium & Serum: See Propagation
Growth Properties: adherent
Organism: Homo sapiens
Morphology: fibroblast
Source: Tissue: connective tissue
Disease: fibrosarcoma
Permits/Forms: In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
Isolation: Temple Texas, United States
Isolation date: 1974
Tumorigenic: Yes
Cytogenetic Analysis: SW 684细胞hypertriploid; modal number = 73; range = 59 to 79. The rate of higher ploidies was 9.1%. Eleven markers were common to most cells. These include: der(2)t(2;6)(p13;q13), der(12)t(8;12)(q11;q24), t(15q21q), 19q+, t(8p21q?) and six others. Of these, the der(2) and t(8p21q?) were generally paired. A few cells had double minutes (DM) (one per cell when present). There were 4 copies of N1, N18, N20 and N22 in most cells. Normal 15 and Y were absent. The X was paired in all cells.
Isoenzymes: AK-1, 1-2
G6PD, B
GLO-I, 2
PGM1, 1-2
PGM3, 1
Age: 68 years
Gender: male
Ethnicity: Caucasian
Propagation: ATCC complete growth medium: The base medium for this cell line is ATCC-formulated Leibovitz's L-15 Medium, Catalog No. 30-2008. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Atmosphere: air, 100%
Temperature: 37.0°C
Subculturing: Protocol:
SW 684细胞Remove and discard culture medium.
Briefly rinse the cell layer with 0.25% (w/v) Trypsin - 0.35 mM EDTA solution to remove all traces of serum which contains trypsin inhibitor.
Add 2.0 to 3.0 ml of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).
Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37C to facilitate dispersal.
Add 6.0 to 8.0 ml of complete growth medium and aspirate cells by gently pipetting.
Add appropriate aliquots of the cell suspension to new culture vessels.
Incubate cultures at 37C.
Subcultivation Ratio: A subcultivation ratio of 1:3 to 1:5 is recommended
Medium Renewal: 2 to 3 times per week
Preservation: Freeze medium: culture medium, 95%; DMSO, 5%
Storage temperature: liquid nitrogen vapor phase
References: 22536: Fogh J, et al. Absence of HeLa cell contamination in 169 cell lines derived from human tumors. J. Natl. Cancer Inst. 58: 209-214, 1977. PubMed: 833871
22539: Fogh J, et al. SW 684细胞One hundred and twenty-seven cultured human tumor cell lines producing tumors in nude mice. J. Natl. Cancer Inst. 59: 221-226, 1977. PubMed: 327080
