D283 Med细胞
年限： 6 years juvenile
细胞形态： 上皮样
数量： 大量
是否是肿瘤细胞： 1
物种来源： 人
器官来源： 大脑
生长状态： 悬浮生长
运输方式： 冻存运输
ATCC Number： HTB-185™
相关**： 其他**
组织来源： cerebellum
Designations: D283 Med
D283 Med细胞Depositors: HS Friedman
Biosafety Level: 1
Shipped: frozen
Medium & Serum: See Propagation
Growth Properties: suspension, multicell aggregates and some adherent cells
Organism: Homo sapiens
Morphology: epithelial
Source: Organ: brain
Tissue: cerebellum
Disease: medulloblastoma
Derived from metastatic site: peritoneum
Cellular Products: neurofilament proteins (NFP)
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Isolation: Isolation date: 1985
Tumorigenic: D283 Med细胞Yes
DNA Profile (STR): Amelogenin: X,Y
CSF1PO: 9,12
D13S317: 8,10
D16S539: 11
D5S818: 11
D7S820: 10
THO1: 7
TPOX: 8,11
vWA: 16,18
Cytogenetic Analysis: The karyotype is 45, XY, -7, -8, -17, -20, der(20)t(1;20)(q12;q13), 8q+, 17p+ (range = 41 to 46). This is a hypodiploid cell line with a frequency of higher ploidies of 5.4%. Three marker chromosomes are present in all cells. They are: der(20)t(1;20)(q12;q13), 8q+ and 17p+. N7, N17 and N20 have single copies. The single X is structurally normal, and the Y chromosome is present as confirmed by fluorescence microscopy.
D283 Med细胞Isoenzymes: AK-1, 1
ES-D, 1
G6PD, B
GLO-I, 2
Me-2, 0
PGM1, 1
PGM3, 1
Age: 6 years juvenile
Gender: male
Ethnicity: Caucasian
Comments: The D283 Med cell line was established in 1985 by Friedman et al. from malignant ascites cells and a peritoneal metastasis from a boy with medulloblastoma. The cells produce tumors in nude mice, and the resulting tumors are positive for expression of neurofibrillary proteins, glutamine synthetase and neuron specific enolase but negative for glial fibrillary acidic proteins and S100 (S-100) protein.
Propagation: ATCC complete growth medium: The base medium for this cell line is ATCC-formulated Eagle's Minimum Essential Medium, Catalog No. 30-2003. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Temperature: 37.0°C
Atmosphere: air, 95%; carbon dioxide (CO2), 5%
Subculturing: Protocol: D283 Med细胞Cultures can be maintained by addition or replacement of medium. Adherent cells can be dislodged by scraping, and cultures can be established by resuspending a cell pellet at 5 X 10(5) viable cells/ml. Maintain cultures between 4 X 10(5) and 1 X 10(6) cells/ml.
Medium Renewal: 2 to 3 times per week
Preservation: Freeze medium: culture medium, 90%; DMSO, 10%
Storage temperature: liquid nitrogen vapor phase
Doubling Time: 52 hrs
Related Products: Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2003
recommended serum:ATCC 30-2020
References: 2096: Friedman HS, et al. Phenotypic and genotypic analysis of a human meduloblastoma cell line and transplantable xenograft (D341 Med) demonstrating amplification of c-myc. Am. J. Pathol. 130: 472-484, 1988. PubMed: 3279793
23092: He XM, et al. Expression of O6-methylguanine-DNA methyltransferase in six human medulloblastoma cell lines. Cancer Res. 52: 1144-1148, 1992. PubMed: 1737373
23157: Friedman HS, et al. Establishment and characterization of the human medulloblastoma cell line and transplantable xenograft D283 Med. J. Neuropathol. Exp. Neurol. 44: 592-605, 1985. PubMed: 4056828
23181: Trojanowski JQ, et al. A rapidly dividing human medulloblastoma cell line (D283 MED) expresses all three neurofilament subunits. Am. J. Pathol. 126: 358-363, 1987. PubMed: 3103453
32287: Rostomily RC, et al. Expression of neurogenic basic helix-loop-helix genes in primitive neuroectodermal tumors. Cancer Res. 57: 3526-3531, 1997. PubMed: 9270024
