Hs 913T细胞
生长状态： 贴壁生长
ATCC Number： HTB-152™
相关**： 纤维肉瘤
数量： 大量
运输方式： 冻存运输
细胞形态： 成纤维样
是否是肿瘤细胞： 1
物种来源： 人
年限： 51 years
Designations: Hs 913T
Depositors: RB Owens
Hs 913T细胞Biosafety Level: 1
Shipped: frozen
Medium & Serum: See Propagation
Growth Properties: adherent
Organism: Homo sapiens
Morphology: fibroblast
Source: Disease: fibrosarcoma
Derived from metastatic site: lung
Permits/Forms: In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
DNA Profile (STR): Amelogenin: X,Y
CSF1PO: 12
D13S317: 11
D16S539: 13
D5S818: 10
Hs 913T细胞D7S820: 10
THO1: 8,9.3
TPOX: 9,11
vWA: 17,18
Cytogenetic Analysis: modal number = 111 to 113; range = 95 to 120; large submetacentric and double minute (DM) markers
Isoenzymes: AK-1, 1
ES-D, 1
G6PD, B
GLO-I, 1
PGM1, 2
PGM3, 2
Hs 913T细胞Age: 51 years
Gender: male
Ethnicity: Caucasian
Propagation: ATCC complete growth medium: The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Subculturing: Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:4 is recommended
Medium Renewal: 2 to 3 times per week
Remove medium, rinse with fresh 0.25% trypsin, 0.02% EDTA solution, remove trypsin and let the culture sit at room temperature (or at 37C) until the cells detach (about 10 minutes).
Add fresh medium, aspirate and dispense into new flasks.
Subculture every 6 to 8 days.
Preservation: Hs 913T细胞Culture medium, 95%; DMSO, 5%
