G-292, clone A141B1细胞
生长状态： 贴壁生长
数量： 大量
是否是肿瘤细胞： 1
物种来源： 人
年限： 9 years
ATCC Number： CRL-1423™
相关**： 骨肉瘤
运输方式： 冻存运输
器官来源： 骨
细胞形态： 成纤维样
G-292, clone A141B1细胞Designations: G-292, clone A141B1
Depositors: PT Peebles
Biosafety Level: 1
Shipped: frozen
Medium & Serum: See Propagation
Growth Properties: adherent
Organism: Homo sapiens
Morphology: fibroblast
Source: Organ: bone
Disease: osteosarcoma
Permits/Forms: In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
Receptors: G-292, clone A141B1细胞epidermal growth factor (EGF)
Isoenzymes: G6PD, B
Age: 9 years
Gender: female
Ethnicity: Caucasian
Propagation: ATCC complete growth medium: The base medium for this cell line is ATCC-formulated McCoy's 5a Medium Modified, Catalog No. 30-2007. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Atmosphere: air, 95%; carbon dioxide (CO2), 5%
Temperature: 37.0°C
Subculturing: Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:4 is recommended
Medium Renewal: G-292, clone A141B1细胞24 hours after seeding from a thawed ampule, then 2 to 3 times per week
Remove medium, add fresh 0.25% trypsin, 0.03% EDTA for several minutes, remove trypsin and let the culture sit at 37C for 10 to 15 minutes. Add fresh medium, aspirate and dispense into new flasks.
References: 22209: . . Pediatr. Res. 12: 485, 1978.
23015: Zhang W, et al. EGF-mediated phosphorylation of extracellular signal-regulated kinases in osteoblastic cells. J. Cell. Physiol. 162: 348-358, 1995. G-292, clone A141B1细胞PubMed: 7860643
