JEG-3细胞
生长状态： 贴壁生长
细胞形态： 上皮样
是否是肿瘤细胞： 1
物种来源： 人
运输方式： 冻存运输
数量： 大量
器官来源： 胎盘
ATCC Number： HTB-36™
相关**： 其他**
Designations: JEG-3
Depositors: G Kohler
JEG-3细胞Biosafety Level: 1
Shipped: frozen
Medium & Serum: See Propagation
Growth Properties: adherent
Organism: Homo sapiens
Morphology: epithelial
Source: Organ: placenta
Disease: choriocarcinoma
Cellular Products: human chorionic gonadotropin (hCG), human chorionic somatomammotropin (placental lactogen); progesterone
Permits/Forms: In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
Applications: transfection host (Roche Transfection Reagents)
Tumorigenic: Yes
DNA Profile (STR): Amelogenin: X,Y
CSF1PO: 11,12
D13S317: 9,11
D16S539: 13,14
D5S818: 10,11
JEG-3细胞D7S820: 10,12
THO1: 9,9.3
TPOX: 8
vWA: 16
Cytogenetic Analysis: This is a hypertriploid human cell line. The modal chromosome number is 71, occurring at 34%, and polyploidy at 2.6%. The t(4;11)(p15;q13), i(13q), t(10p15q), del(18)(q21), and 6 other markers are common to most cells, and two other markers are found in some. Giant satellites are seen in one N14, and two N22. N2, N5, and N9 have 4 copies, and N7, N13, N18, N21 and X a single copy. A single Y chromosome is detected by Q-band examination.
Isoenzymes: AK-1, 1
ES-D, 1
G6PD, B
GLO-I, 1-2
PGM1, 1
PGM3, 1-2
Comments: This is one of six clonally derived lines isolated from the Woods strain of the Erwin-Turner tumor by Kohler and associates.
The cells are able to transform steroid precursors to estrone and estradiol
Propagation: ATCC complete growth medium: The base medium for this cell line is ATCC-formulated Eagle's Minimum Essential Medium, Catalog No. 30-2003. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Temperature: 37.0°C
Subculturing: JEG-3细胞Subcultivation Ratio: A subcultivation ratio of 1:4 to 1:6 is recommended
Medium Renewal: 2 to 3 times per week
Remove medium, and rinse with 0.25% trypsin, 0.03% EDTA solution. Remove the solution and add an additional 1 to 2 ml of trypsin-EDTA solution. Allow the flask to sit at room temperature (or at 37C) until the cells detach.
Add fresh culture medium, aspirate and dispense into new culture flasks.
Preservation: Freeze medium: Culture medium, 95%; DMSO, 5%
Storage temperature: liquid nitrogen vapor phase
Related Products: Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2003
recommended serum:ATCC 30-2020
References: 22536: Fogh J, et al. Absence of HeLa cell contamination in 169 cell lines derived from human tumors. J. Natl. Cancer Inst. 58: 209-214, 1977. PubMed: 833871
22539: Fogh J, et al. One hundred and twenty-seven cultured human tumor cell lines producing tumors in nude mice. J. Natl. Cancer Inst. 59: 221-226, 1977. PubMed: 327080
23377: . . Acta Endocrinol. Suppl. 153: 137-153, 1971.
32288: Landers JE, et al. Translational enhancement of mdm2 oncogene expression in human tumor cells containing a stabilized wild-type p53 protein. Cancer Res. 57: 3562-3568, 1997. PubMed: 9270029
32564: Roesler WJ, et al. The alpha-isoform of the CCAAT/enhancer-binding protein is required for mediating cAMP responsiveness of the phosphoenolpyruvate carboxykinase promoter in hepatoma cells. J. Biol. Chem. 271: 8068-8074, 1996. PubMed: 8626491
58051: Kohler PO, Bridson WE. JEG-3细胞Isolation of hormone-producing clonal lines of human choriocarcinoma. J. Clin. Endocrinol. 32: 683-687, 1971.
