DMS 114细胞
是否是肿瘤细胞： 1
物种来源： 人
器官来源： 肺
运输方式： 冻存运输
ATCC Number： CRL-2066™
相关**： 小细胞肺癌
年限： 68 years
数量： 大量
生长状态： 贴壁生长
Designations: DMS 114
Depositors: OS Pettengill, G Sorenson
DMS 114细胞Biosafety Level: 1
Shipped: frozen
Medium & Serum: See Propagation
Growth Properties: adherent
Organism: Homo sapiens
Morphology:
Source: Organ: lung
Disease: carcinoma; small cell lung cancer
Cellular Products: adrenocorticotropin (adrenocorticotropic hormone, ACTH); bombesin; glucagon; 17 beta estradiol; oxytocin - neurophysin (OT-NP)
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Receptors: epidermal growth factor (EGF); complement (CR3)
Tumorigenic: Yes
Antigen Expression: Leu 7 +; My23 +; CD11b +
DNA Profile (STR): Amelogenin: X
CSF1PO: 10,11
DMS 114细胞D13S317: 13
D16S539: 12
D5S818: 12
D7S820: 10,11
THO1: 8,9.3
TPOX: 8,11
vWA: 16,17
Age: 68 years
Gender: male
Ethnicity: Caucasian
Comments: The line was established from cells from a mediastinal biopsy of a patient with small cell carcinoma of the lung.
The patient had not received prior therapy.
DMS 114细胞The cells express HLA class I and class II antigens.
Early passages of the cells were contaminated with a bovine mycoplasma (Acholeplasma laidlawii) which was cured (prior to cryopreservation) with A. laidlawii antiserum and kanamycin.
Propagation: ATCC complete growth medium: Waymouth's MB 752/1 medium, 90%; fetal bovine serum, 10%
Temperature: 37.0°C
Subculturing: Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:4 is recommended
Medium Renewal: Twice per week
Remove medium, and rinse with 0.25% trypsin, 0.03% EDTA solution. Remove the solution and add an additional 1 to 2 ml of trypsin-EDTA solution. Allow the flask to sit at room temperature (or at 37C) until the cells detach.
Add fresh culture medium, aspirate and dispense into new culture flasks.
Preservation: Culture medium, 95%; DMSO, 5%
Related Products: recommended serum:ATCC 30-2020
References: 22793: Pettengill OS, et al. Isolation and growth characteristics of continuous cell lines from small-cell carcinoma of the lung. Cancer 45: 906-918, 1980. PubMed: 6266631
23142: Pettengill OS, et al. Animal model for small cell carcinoma of the lung. Effect of immunosuppression and sex of mouse on tumor growth in nude athymic mice. Exp. Cell Biol. 48: 279-297, 1980. DMS 114细胞PubMed: 6249655
