BC-1细胞
运输方式： 冻存运输
生长状态： 悬浮生长
年限： 46 years
ATCC Number： CRL-2230™
相关**： **瘤
数量： 大量
细胞形态： **样
细胞类型： B**细胞
是否是肿瘤细胞： 1
物种来源： 人
BC-1细胞Designations: BC-1
Depositors: E Cesarman
Biosafety Level: 2 [CELLS CONTAIN HERPESVIRUS ]
Shipped: frozen
Medium & Serum: See Propagation
Growth Properties: suspension
Organism: Homo sapiens
Morphology: lymphoblast
Source: Disease: lymphoma
Cell Type: B lymphocyte;
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Applications: transfection host (Roche Transfection Reagents)
Antigen Expression: CD30 +; CD45 +; EMA + (epithelial membrane antigen)
DNA Profile (STR): BC-1细胞Amelogenin: X,Y
CSF1PO: 10,11
D13S317: 8
D16S539: 12,13
D5S818: 11,12
D7S820: 11
THO1: 9.3
TPOX: 8,9
vWA: 16,20
Age: 46 years
Gender: male
Comments: BC-1 is a lymphoma cell line originated in 1992 by Ethel Cesarman and Giorgio Inghirami in the laboratory of Daniel M. Knowles.
The cells were derived from an acquired immunodeficiency syndrome (AIDS) related body cavity based lymphoma presenting as a primary lymphomatous effusion in the peritoneal cavity.
The cells contain two viral genomes: BC-1细胞Epstein-Barr virus (EBV) and Kaposi sarcoma associated herpesvirus (KSHV, provisionally designed HHV-8).
KSHV is a recently identified and largely uncharacterized virus.
The BC-1 cell line allows in vitro culture of KSHV viral genomes.
It is also a practical source of KSHV DNA to be used as a positive control for screening tests.
Propagation: ATCC complete growth medium: RPMI 1640 medium, 80%; fetal bovine serum, 20%
Subculturing: Medium Renewal: Every 2 to 3 days
Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 5 X 10 exp5 cells/ml and maintain between 5 X 10 exp5 and 2 X 10 exp6 cells/ml.
Preservation: Culture medium, 95%; DMSO, 5%
Doubling Time: 48 to 72 hrs
References: 22572: Moore PS, et al. Primary characterization of a herpesvirus agent associated with Kaposi's sarcoma. J. Virol. 70: 549-558, 1996. PubMed: 8523568
23358: Cesarman E, et al. In vitro establishment and characterization of two acquired immunodeficiency syndrome-related lymphoma cell lines (BC-1 and BC-2) containing Kaposi's sarcoma-associated herpesvirus-like (KSHV) DNA sequences. Blood 86: 2708-2714, 1995. PubMed: 7670109
23395: Chadburn A, et al. BC-1细胞CD30 (Ki-1) positive anaplastic large cell lymphomas in individuals infected with the human immunodeficiency virus. Cancer 72: 3078-3090, 1993. PubMed: 8221575
23493: Cesarman E, et al. Kaposi's sarcoma-associated herpesvirus-like DNA sequences in AIDS- related body-cavity-based lymphomas. N. Engl. J. Med. 332: 1186-1191, 1995. PubMed: 7700311
