NCI-H1793细胞
运输方式： 冻存运输
年限： 52 years
器官来源： 肺
是否是肿瘤细胞： 1
物种来源： 人
数量： 大量
生长状态： 贴壁生长
ATCC Number： CRL-5896™
相关**： 非小细胞肺癌
细胞形态： 上皮样
Designations: NCI-H1793 [H1793]
Depositors: AF Gazdar, JD Minna
Biosafety Level: 1
NCI-H1793细胞Shipped: frozen
Medium & Serum: See Propagation
Growth Properties: adherent
Organism: Homo sapiens
Morphology: epithelial
Source: Organ: lung
Disease: adenocarcinoma; non-small cell lung cancer
Permits/Forms: In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
Restrictions: The line is available with the following restrictions: 1. This cell line was deposited at the ATCC by Dr. A. Gazdar and Dr. J. Minna and is provided for research purposes only. Neither the cell line nor products derived from it may be sold or used for commercial purposes. Nor can the cells be distributed to third parties for purposes of sale, or producing for sale, cells or their products. The cells are provided as service to the research community. They are provided without warranty of merchantability or fitness for a particular purpose or any other warranty, expressed or implied. 2. Any proposed commercial use of the these cells, or their products must first be negotiated with the University of Texas Southwestern Medical Center at Dallas, 5323 Harry Hines Blvd., Dallas, Texas 75235. Telephone (214) 699-8056, FAX (214) 688-7233.
DNA Profile (STR): Amelogenin: X
CSF1PO: 9,12
D13S317: 8,12
D16S539: 13
NCI-H1793细胞D5S818: 11,12
D7S820: 7,8
THO1: 9,9.3
TPOX: 8
vWA: 18
Age: 52 years
Gender: female
Comments: The line was established in December 1987.
The tissue donor was a non-smoker.
Propagation: ATCC complete growth medium: HITES medium supplemented with 5% fetal bovine serum
NCI-H1793细胞The base medium for this cell line is ATCC-formulated DMEM:F12 Medium Catalog No.30-2006. To make the complete growth medium,add the following components to the base medium
0.005 mg/ml Insulin
0.01 mg/ml Transferrin
30nM Sodium selenite (final conc.)
10 nM Hydrocortisone (final conc.)
10 nM beta-estradiol (final conc.)
extra 2mM L-glutamine (for final conc. of 4.5 mM)
5% fetal bovine serum (final conc.)
Temperature: 37.0°C
Subculturing: Subcultivation Ratio: NCI-H1793细胞A subcultivation ratio of 1:3 to 1:6 is recommended
Remove medium, and rinse with 0.25% trypsin, 0.03% EDTA solution. Remove the solution and add an additional 1 to 2 ml of trypsin-EDTA solution. Allow the flask to sit at room temperature (or at 37C) until the cells detach.
Add fresh culture medium, aspirate and dispense into new culture flasks.
Preservation: Culture medium, 95%; DMSO, 5%
Related Products: Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2006
recommended serum:ATCC 30-2020
References: 23570: . NCI-Navy Medical Oncology Branch Cell Line Supplement. J. Cell. Biochem. suppl. 24: 1996.
