EB1细胞
年限： 9 years
细胞形态： **样
ATCC Number： HTB-60™
相关**： **瘤
组织来源： upper maxilla
细胞类型： B**细胞
是否是肿瘤细胞： 1
物种来源： 人
数量： 大量
生长状态： 悬浮生长
运输方式： 冻存运输
EB1细胞Designations: EB1
Depositors: G Trempe, LJ Old
Biosafety Level: 2 [Cells Contain HERPESVIRUS ]
Shipped: frozen
Medium & Serum: See Propagation
Growth Properties: suspension
Organism: Homo sapiens
Morphology: lymphoblast
Source: Tissue: upper maxilla
Disease: Burkitt's lymphoma
Cell Type: B lymphocyte;
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Isoenzymes: ES-D, 1
G6PD, B
GLO-I, 2
PGM1, 1
Age: EB1细胞9 years
Gender: female
Ethnicity: Black
Comments: The EB1 line was isolated in 1963 by M.A. Epstein and Y.M. Barr from biopsy fragments and cell clumps of the lymphoma.
Viral particles (Epstein-Barr virus) were noted through electron microscopic examination.
Propagation: ATCC complete growth medium: The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, Catalog No. 30-2001. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Subculturing: Subcultivation Ratio: A subcultivation ratio of 1:2 is recommended
Medium Renewal: Twice per week
Allow cells to settle, remove most of the supernatant medium, dilute with fresh medium to 4 X 10 exp5 viable cell/ml. Subculture at time of fluid renewal.
Preservation: Culture medium, 95%; DMSO, 5%
References: 22143: Epstein MA, Barr YM. Cultivation in vitro of human lymphoblasts from Burkitt's malignant lymphoma. Lancet 1: 252-253, 1964. PubMed: 14090852
22169: Epstein MA, Barr YM. EB1细胞Characteristics and mode of growth of tissue culture strain (EB1) of human lymphoblasts from Burkitt's lymphoma. J. Natl. Cancer Inst. 34: 231-240, 1965. PubMed: 14293790
22722: . . Br. J. Cancer 19: 108-115, 1965.
23216: Giraldo G, et al. Kaposi's sarcoma: a new model in the search for viruses associated with human malignancies. J. Natl. Cancer Inst. 49: 1495-1507, 1972. PubMed: 4346014
26323: . . J. Exp. Med. 121: 761-770, 1965.
26324: Leder P, et al. Translocations among antibody genes in human cancer. Science 222: 765, 1983. PubMed: 6356357
26325: Dalla-Favera R, et al. Human c-myc onc gene is located on the region of chromosome 8 that is translocated in Burkitt lymphoma cells. Proc. Natl. Acad. Sci. USA 79: 7824-7827, 1982. PubMed: 6961453
26326: Taub R, et al. Translocation of the c-myc gene into the immunoglobulin heavy chain locus in human Burkitt lymphoma and murine plasmacytoma cells. Proc. Natl. Acad. Sci. USA 79: 7837-7841, 1982. EB1细胞PubMed: 6818551
