CHL-1细胞
数量： 大量
器官来源： 皮肤
运输方式： 冻存运输
细胞形态： 上皮样
ATCC Number： CRL-9446™
相关**： 黑色素瘤
生长状态： 贴壁生长
是否是肿瘤细胞： 1
物种来源： 人
Designations: CHL-1
CHL-1细胞Depositors: Berlex Laboratories, Inc.
Biosafety Level: 1
Shipped: frozen
Medium & Serum: See Propagation
Growth Properties: adherent
Organism: Homo sapiens
Morphology: epithelial
Source: Organ: skin
Disease: melanoma
Cellular Products: human tissue plasminogen activator (t-PA)
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Applications: CHL-1细胞transfection host
Transfection host derived from RPMI 7932 that can grow to a higher density, namely 5 x 10(7) cells/ml. [41429]
DNA Profile (STR): Amelogenin: X
CSF1PO: 10
D13S317: 8,11
D16S539: 13
D5S818: 11,12
D7S820: 11,13
THO1: 9,9.3
TPOX: 8
vWA: 17
Comments: This is a derivative of the RPMI 7932 human melanoma cell line (Bowes).
It produces about 0.1 mU of t-PA per cell per day.
Propagation: ATCC complete growth medium: The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium, Catalog No. 30-2002. CHL-1细胞To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Temperature: 37.0°C
Subculturing: Protocol: Remove medium, and rinse with 0.25% trypsin, 0.03% EDTA solution. Remove the solution and add an additional 1 to 2 ml of trypsin-EDTA solution. Allow the flask to sit at room temperature (or at 37C) until the cells detach. Add fresh culture medium, aspirate and dispense into new culture flasks.
Subcultivation Ratio: A subcultivation ratio of 1:6 to 1:10 is recommended
Medium Renewal: Add fresh medium at the time of subculture
Preservation: Freeze medium: Complete growth medium, 95%; DMSO, 5%
Storage temperature: liquid nitrogen vapor temperature
Related Products: Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2002
recommended serum:ATCC 30-2020
References: 41429: Cashion L, et al. CHL-1细胞Transfected cells containing plasmids having genes oriented in opposing directions and methods of using. US Patent 5,017,478 dated May 21 1991
