NCI-H128细胞
是否是肿瘤细胞： 1
物种来源： 人
年限： 60 years
数量： 大量
生长状态： 漂浮生长
细胞形态： 其他
ATCC Number： HTB-120™
相关**： 小细胞肺癌
器官来源： 肺
运输方式： 冻存运输
Designations: NCI-H128 [H128]
Depositors: AF Gazdar
NCI-H128细胞Biosafety Level: 1
Shipped: frozen
Medium & Serum: See Propagation
Growth Properties: floating aggregates
Organism: Homo sapiens
Morphology: floating aggregates
Source: Organ: lung
Disease: carcinoma; small cell lung cancer
Derived from metastatic site: pleural effusion
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Tumorigenic: Yes
DNA Profile (STR): Amelogenin: X
CSF1PO: 10
D13S317: 12
D16S539: 13
D5S818: 9
D7S820: 8,10
THO1: 6,9
TPOX: 8
vWA: 17
Cytogenetic Analysis: NCI-H128细胞There are 2 distinct aneuploid peaks, both have characteristic 3p deletion.
Isoenzymes: AK-1, 1
ES-D, 1
G6PD, A
GLO-I, 1-2
Me-2, 1
PGM1, 1
PGM3, 1
Age: 60 years
Gender: male
Ethnicity: Black
Comments: This cell line is aneuploid.
Will form colonies in soft agar.
It retains small cell carcinoma morphology and ultrastructure as well as APUD cell characteristics.
NCI-H128 cells do well on a rotary shaker flask at 70 to 80 rpm at 37C.
It is normal for cultures of this line to have fairly large amounts of cell debris.
Propagation: ATCC complete growth medium: RPMI 1640 medium, 80%; fetal bovine serum, 20% - OR - Iscove's modified Dulbecco's medium, 80%; fetal bovine serum, 20%
Subculturing: Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:4 is recommended
Medium Renewal: Twice per week
NCI-H128细胞Allow cell aggregates to settle to the bottom of the flask, discard the supernatant medium, disperse the cells with gentle pipetting and dispense into new flasks. Subculture every 6 to 8 days.
Preservation: Culture medium, 90%; DMSO, 10%
Related Products: normal (or near-normal) cell line established from the same patient:ATCC CRL-5947
References: 1805: Little CD, et al. Amplification and expression of the c-myc oncogene in human lung cancer cell lines. Nature 306: 194-196, 1983. PubMed: 6646201
23036: Gazdar AF, et al. Establishment of continuous, clonable cultures of small-cell carcinoma of lung which have amine precursor uptake and decarboxylation cell properties. Cancer Res. 40: 3502-3507, 1980. PubMed: 6108156
23037: . . Cancer Res. 40: 4556-4563, 1980.
23056: Carney DN, et al. Establishment and identification of small cell lung cancer cell lines having classic and variant features. Cancer Res. 45: 2913-2923, 1985. PubMed: 2985257
23057: Gazdar AF, et al. Characterization of variant subclasses of cell lines derived from small cell lung cancer having distinctive biochemical, morphological, and growth properties. Cancer Res. 45: 2924-2930, 1985. PubMed: 2985258
23080: Hensel CH, et al. Altered structure and expression of the human retinoblastoma susceptibility gene in small cell lung cancer. Cancer Res. 50: 3067-3072, 1990. PubMed: 2159370
32276: Cairns P, et al. NCI-H128细胞Genomic organization and mutation analysis of Hel-N1 in lung cancers with chromosome 9p21 deletions. Cancer Res. 57: 5356-5359, 1997. PubMed: 9393760
