SW 1783细胞
器官来源： 大脑
年限： grade III
是否是肿瘤细胞： 1
物种来源： 人
生长状态： 贴壁生长
ATCC Number： HTB-13™
相关**： 其他**
运输方式： 冻存运输
数量： 大量
细胞形态： 成纤维样
Designations: SW 1783 [SW-1783, SW1783]
Depositors: A Leibovitz
SW 1783细胞Biosafety Level: 1
Shipped: frozen
Medium & Serum: See Propagation
Growth Properties: adherent
Organism: Homo sapiens
Morphology: fibroblast
Source: Organ: brain
Tumor Stage: grade III
Disease: astrocytoma
Permits/Forms: In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
Isolation: Temple Texas, United States
Isolation date: 1977
DNA Profile (STR): Amelogenin: X,Y
CSF1PO: 10,13
SW 1783细胞D13S317: 9,11
D16S539: 12
D5S818: 12,13
D7S820: 9,13
THO1: 6,8
TPOX: 8,11
vWA: 16,17
Cytogenetic Analysis: hypertetraploid; modal number = 96. The rate of higher ploidies was 10.1%. The markers der(5)t(1;5) (p11;p15), t(1q3q), del(3) (q21), der(6)t(6;11) (p12;p15), der(11)t(6;11) (p12;p15), and 5 others were common to most cells. Of these, der (6) and der(11) had consistently two copies per cell, and were probably formed by balanced translocation between N6 and N11. Nine to 10 marker chromosomes were common to some cells, and 10 to 15 others were detected only once. All normal chromosomes were present in 2 to 5 copies per cell. Generally X had 3 copies and Y had 2 copies per cell. Double minutes (DM) were seen in some cells, and only 1 to a few copies occurred per cell.
Isoenzymes: AK-1, 1
ES-D, 1
G6PD, B
GLO-I, 2
PGM1, 1
PGM3, 1
Age: SW 1783细胞68 years *****
Gender: male
Ethnicity: Caucasian
Propagation: ATCC complete growth medium: The base medium for this cell line is ATCC-formulated Leibovitz's L-15 Medium, Catalog No. 30-2008. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Atmosphere: air, 100%
Temperature: 37.0°C
Subculturing: Protocol: Remove medium, rinse with fresh 0.25% trypsin solution, remove trypsin and let the culture sit at room temperature (or at 37C) until the cells detach (about 10 minutes). Add fresh medium, aspirate and dispense into new flasks.
Interval: every 6 to 8 days
Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:4 is recommended
Medium Renewal: 2 to 3 times per week
References: 32274: Koochekpour S, et al. SW 1783细胞Met and hepatocyte growth factor/scatter factor expression in human gliomas. Cancer Res. 57: 5391-5398, 1997. PubMed: 9393765
