MNNG/HOS (Cl #5)细胞
年限： 13 years
是否是肿瘤细胞： 1
物种来源： 人
数量： 大量
运输方式： 冻存运输
器官来源： 骨
ATCC Number： CRL-1547™
相关**： 骨肉瘤
生长状态： 贴壁生长
Designations: MNNG/HOS Cl #5 [R-1059-D]
Depositors: JS Rhim
MNNG/HOS (Cl #5)细胞Biosafety Level: 1
Shipped: frozen
Medium & Serum: See Propagation
Growth Properties: adherent
Organism: Homo sapiens
Morphology:
Source: Organ: bone
Disease: osteosarcoma
Permits/Forms: In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
Tumorigenic: Yes
DNA Profile (STR): Amelogenin: X
CSF1PO: 12
MNNG/HOS (Cl #5)细胞D13S317: 12
D16S539: 10,13
D5S818: 13
D7S820: 11,12
THO1: 6
TPOX: 8,11
vWA: 18
Isoenzymes: G6PD, B
Age: 13 years
Gender: female
Ethnicity: Caucasian
Comments: This line was derived from HOS (ATCC CRL-1543) by transformation with 0.01 mcg/ml MNNG (a carcinogenic nitrosamine). The cells exhibit a high saturation density, a high plating efficiency in soft agar and are tumorigenic in nude mice.
Propagation: MNNG/HOS (Cl #5)细胞ATCC complete growth medium: The base medium for this cell line is ATCC-formulated Eagle's Minimum Essential Medium, Catalog No. 30-2003. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Subculturing: Subcultivation Ratio: A subcultivation ratio of 1:4 is recommended
Medium Renewal: 2 to 3 times per week
Remove medium, rinse with fresh 0.25% trypsin solution, remove trypsin and let the culture sit at room temperature for 5 to 10 minutes. Add fresh medium, aspirate and dispense into new flasks. Subculture every 2 to 5 days.
References: 22895: Rhim JS, et al. Transformation of human cells in culture by N-methyl-N'-nitro-N- nitrosoguanidine. Nature 256: 751-753, 1975. PubMed: 1057046
26084: Rhim JS, et al. Characterization of human cells transformed in vitro by N-methyl-N'- nitro-N-nitrosoguanidine. Int. J. Cancer 19: 505-510, 1977. PubMed: 265298
32925: Zhu X, et al. Cell cycle-dependent modulation of telomerase activity in tumor cells. Proc. MNNG/HOS (Cl #5)细胞Natl. Acad. Sci. USA 93: 6091-6095, 1996. PubMed: 8650224
