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SW756细胞

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  • 产品名称:SW756细胞
  • 产品型号:SW756
  • 产品展商:HZbscience
  • 产品文档:无相关文档
  • 发布时间:2018-06-26
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简单介绍
SW756细胞应如何避免细胞污染,细胞污染的种类可分成**、酵母菌、霉菌、病毒和霉浆菌。主要的污染原因为无菌操作技术不当、操作室环境不佳、污染之血清和污染之细胞等。严格之无菌操作技术、清洁的环境、与品质良好之细胞来源和培养基配制是减低污染之*好方法。SW756细胞何时须更换培养基?视细胞生长密度而定,或遵照细胞株基本数据上之更换时间,按时更换培养基即可。
产品描述

SW756细胞

ATCC Number: CRL-10302™

相关**: 鳞状细胞癌

数量: 大量

器官来源: 宫颈

是否是肿瘤细胞: 1

物种来源: 人

细胞形态: 上皮样

运输方式: 冻存运输

生长状态: 贴壁生长

年限: 46 years

Designations: SW756

Depositors: Univ. Texas System, Board of Regents

SW756细胞Biosafety Level: 1

Shipped: frozen

Medium & Serum: See Propagation

Growth Properties: adherent

Organism: Homo sapiens

Morphology: epithelial


Source: Organ: cervix

Disease: squamous cell carcinoma

Permits/Forms: In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.

Isolation: Temple Texas, United States

Isolation date: 1974

Virus Susceptibility: Influenza A virus

Tumorigenic: Yes

Antigen Expression: SW756细胞HLA A1, A24, B8, B44, Cw2, Cx, DR6Y; Le3; Le4; Le5

DNA Profile (STR): Amelogenin: X

CSF1PO: 11,12

D13S317: 11

D16S539: 12,13

D5S818: 11,12

D7S820: 10,12

THO1: 9.3

TPOX: 8

vWA: 17

Cytogenetic Analysis: modal number = 76; range = 60 to 96

Isoenzymes: G6PD, B

GLO-I, 1-2

Age: 46 years

Gender: female

Ethnicity: Caucasian

Propagation: ATCC complete growth medium: The base medium for this cell line is ATCC-formulated Leibovitz's L-15 Medium, Catalog No. 30-2008. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.

Atmosphere: air, 100%

SW756细胞Temperature: 37.0°C

Subculturing: Protocol: Remove medium, rinse with fresh 0.25% trypsin solution, remove trypsin and let the culture sit at room temperature until the cells detach. Add fresh medium, aspirate and dispense into new flasks.

Subcultivation Ratio: A subcultivation ratio of 1:3 to 1:6 is recommended

Medium Renewal: 2 to 3 times per week

Preservation: Freeze medium: culture medium, 95%; DMSO, 5%

Storage temperature: liquid nitrogen vapor phase

References: 22075: Freedman RS, et al. Monoclonal antibody to a human MDR1 multidrug resistance gene product, and uses. US Patent 5,434,076 dated Jul 18 1995

22667: Freedman RS, et al. SW756细胞Characterization of a cell line (SW756) derived from a human squamous carcinoma of the uterine cervix. In Vitro 18: 719-726, 1982. PubMed: 7129487

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