CHP-212细胞
是否是肿瘤细胞： 1
物种来源： 人
数量： 大量
生长状态： 贴壁生长
运输方式： 冻存运输
细胞形态： 神经母细胞
ATCC Number： CRL-2273™
相关**： 神经母细胞瘤
器官来源： 大脑
Designations: CHP-212
Depositors: JL Biedler
CHP-212细胞Biosafety Level: 1
Shipped: frozen
Medium & Serum: See Propagation
Growth Properties: adherent
Organism: Homo sapiens
Morphology: neuroblast
Source: Organ: brain
Disease: neuroblastoma
Permits/Forms: CHP-212细胞In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
DNA Profile (STR): Amelogenin: X,Y
CSF1PO: 12
D13S317: 8,13
D16S539: 13
D5S818: 10,12
D7S820: 11
THO1: 6
TPOX: 8,11
vWA: 15,18
Propagation: CHP-212细胞ATCC complete growth medium: The base medium for this cell line is a 1:1 mixture of ATCC-formulated Eagle's Minimum Essential Medium, Catalog No. 30-2003, and F12 Medium. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Temperature: 37.0°C
Subculturing: Protocol:
Remove and discard culture medium.
Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.
Add 2.0 to 3.0 ml of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).
Note: CHP-212细胞To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37�C to facilitate dispersal.
Add 6.0 to 8.0 ml of complete growth medium and aspirate cells by gently pipetting.
Add appropriate aliquots of the cell suspension to new culture vessels.
Incubate cultures at 37�C.
Subcultivation Ratio: A subcultivation ratio of 1:10 to 1:50 is recommended every 2 to 3 weeks
Medium Renewal: Every 2 to 3 days
Preservation: Freeze medium: Complete growth medium supplemented with 5% (v/v) DMSO
Storage temperature: liquid nitrogen vapor phase
Related Products: recommended serum:ATCC 30-2020
