C2BBe1细胞
是否是肿瘤细胞： 1
物种来源： 人
数量： 大量
生长状态： 贴壁生长
ATCC Number： CRL-2102™
相关**： 大肠癌
年限： 72 years
运输方式： 冻存运输
细胞形态： 上皮样
器官来源： 结肠
Designations: C2BBe1 [clone of Caco-2 (ATCC HTB-37)]
Depositors: MD Peterson, M Mooseker
Biosafety Level: 1
Shipped: frozen
C2BBe1细胞Medium & Serum: See Propagation
Growth Properties: adherent
Organism: Homo sapiens
Morphology: epithelial
Source: Organ: colon
Disease: colorectal adenocarcinoma
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Applications: C2BBe1 cells form a polarized monolayer with an apical brush border (BB) morphologically comparable to that of the human colon.
Isolated BB contained the microvillar proteins villin, fimbrin, sucrase-isomaltase, BB myosin-1 and the terminal web proteins fodrin and myosin II.
The cells express substantial levels of BB myosin I similar to that of the human enterocyte.
Although clonal, and far more homogeneous than the parental Caco-2 cell line with respect to BB expression, these cells are still heterogenous for microvillar length, microvillar aggregation, and levels of expression of certain BB proteins.
Receptors: epidermal growth factor (EGF)
DNA Profile (STR): Amelogenin: X
CSF1PO: 11
D13S317: 11, 13, 14
D16S539: 12, 13
D5S818: 12, 13
C2BBe1细胞D7S820: 11, 12
THO1: 6
TPOX: 9, 11
vWA: 16, 18
Age: 72 years
Gender: male
Ethnicity: Caucasian
Comments: The C2BBe1 (brush border expressing) cell line was cloned in 1988 from the Caco-2 cell line (see ATCC HTB-37) by limiting dilution.
The clone was selected on the basis of morphological homogeneity and exclusive apical villin localization.
C2BBe1 cells form a polarized monolayer with an apical brush border (BB) morphologically comparable to that of the human colon.
Isolated BB contained the microvillar proteins villin, fimbrin, sucrase-isomaltase, BB myosin-1 and the terminal web proteins fodrin and myosin II.
The cells express substantial levels of BB myosin I similar to that of the human enterocyte.
Although clonal, and far more homogeneous than the parental Caco-2 cell line with respect to BB expression, these cells are still heterogenous for microvillar length, microvillar aggregation, and levels of expression of certain BB proteins.
Propagation: ATCC complete growth medium: The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: 0.01 mg/ml human transferrin; fetal bovine serum to a final concentration of 10%.
Temperature: 37.0°C
Subculturing: C2BBe1细胞Protocol:
Remove and discard culture medium.
Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.
Add 2.0 to 3.0 ml of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).
Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37�C to facilitate dispersal.
Add 6.0 to 8.0 ml of complete growth medium and aspirate cells by gently pipetting.
Add appropriate aliquots of the cell suspension to new culture vessels.
Incubate cultures at 37�C.
Subculture at 85% of confluence (about every 5 to 7 days).
Subcultivation Ratio: A subcultivation ratio of 1:6 to 1:10 is recommended
Medium Renewal: Twice per week
Preservation: Freeze medium: Complete growth medium, 95%; DMSO, 5%
Storage temperature: liquid nitrogen vapor phase
Related Products: Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2002
recommended serum:ATCC 30-2020
parental cell line:ATCC HTB-37
References: 23012: Basson MD, et al. C2BBe1细胞Effect of tyrosine kinase inhibition on basal and epidermal growth factor-stimulated human Caco-2 enterocyte sheet migration and proliferation. J. Cell. Physiol. 160: 491-501, 1994. PubMed: 8077287
23149: Peterson MD, Mooseker MS. Characterization of the enterocyte-like brush border cytoskeleton of the C2BBe clones of the human intestinal cell line, Caco-2. J. Cell Sci. 102: 581-600, 1992. PubMed: 1506435
