NCI-H1688细胞
是否是肿瘤细胞： 1
物种来源： 人
ATCC Number： CCL-257™
相关**： 小细胞肺癌
生长状态： 贴壁生长
数量： 大量
年限： 50 years
细胞形态： 上皮样
器官来源： 肺
运输方式： 冻存运输
Designations: NCI-H1688 [H1688]
NCI-H1688细胞Depositors: AF Gazdar, JD Minna
Biosafety Level: 1
Shipped: frozen
Medium & Serum: See Propagation
Growth Properties: adherent
Organism: Homo sapiens
Morphology: epithelial
Source: Organ: lung
Disease: carcinoma; classic small cell lung cancer
Permits/Forms: In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
DNA Profile (STR): Amelogenin: X
CSF1PO: 12
NCI-H1688细胞D13S317: 8
D16S539: 13
D5S818: 10
D7S820: 12
THO1: 6,8
TPOX: 11
vWA: 17
Age: 50 years
Gender: male
Ethnicity: Caucasian
Comments: This line was derived from a liver metastasis taken from a patient prior to therapy.
Propagation: ATCC complete growth medium: The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, Catalog No. 30-2001. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Temperature: 37.0°C
Atmosphere: air, 95%; NCI-H1688细胞carbon dioxide (CO2), 5%
Subculturing: Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:6 is recommended
Medium Renewal: Every 2 to 3 days
Remove spent medium, and add fresh 0.25% trypsin, 0.03% EDTA solution for 2 to 3 minutes at room temperature.
Remove the trypsin and incubate the flask at 37C for 5 to 10 minutes or until the cells detach.
Add fresh medium, aspirate and dispense into new flasks.
Preservation: Culture medium, 95%; DMSO, 5%
Related Products: Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2001
recommended serum:NCI-H1688细胞ATCC 30-2020
