BT-483细胞
年限： 23 years
细胞形态： 上皮样
器官来源： **
运输方式： 冻存运输
生长状态： 贴壁生长
ATCC Number： HTB-121™
相关**： 导管癌
数量： 大量
是否是肿瘤细胞： 1
物种来源： 人
BT-483细胞Designations: BT-483
Depositors: EY Lasfargues
Biosafety Level: 1
Shipped: frozen
Medium & Serum: See Propagation
Growth Properties: adherent
Organism: Homo sapiens
Morphology: epithelial
Source: Organ: mammary gland; breast
Disease: ductal carcinoma
Permits/Forms: In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. BT-483细胞Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
Tumorigenic: Yes
DNA Profile (STR): Amelogenin: X
CSF1PO: 9,12
D13S317: 8,12
D16S539: 11,12
D5S818: 11
D7S820: 9,10
THO1: 5,9.3
TPOX: 8,11
vWA: 17
Cytogenetic Analysis: modal number = 72; range = 46 to 130
Isoenzymes: AK-1, 1
BT-483细胞ES-D, 1-2
G6PD, B
GLO-I, 2
Me-2, 0
PGM1, 2
PGM3, 2
Age: 23 years
Gender: female
Ethnicity: Caucasian
Comments: The cells were highly pleomorphic with prominent nuclei and nucleoli [PubMed ID: 212572]. Epithelial characteristics including desmosomes, microvilli, tight and gap junctions were observed by transmission electron microscopy [PubMed ID: 212572].
Propagation: ATCC complete growth medium: The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, Catalog No. 30-2001. To make the complete growth medium, add the following components to the base medium: 0.01 mg/ml bovine insulin; fetal bovine serum to a final concentration of 20%.
Atmosphere: air, 95%; carbon dioxide (CO2), 5%
Temperature: 37.0°C
Subculturing: Protocol: BT-483细胞Remove medium, add fresh 0.25% trypsin, 0.53 mM EDTA solution for 3 to 5 minutes, remove trypsin and let the culture sit at 37C for 3 to 5 minutes. Add fresh medium, aspirate and dispense into new flasks.
Subcultivation Ratio: A subcultivation ratio of 1:2 is recommended
Medium Renewal: 2 to 3 times per week
Preservation: Freeze medium: Complete growth medium, 95%; DMSO, 5%
Storage temperature: liquid nitrogen vapor temperature
Related Products: Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2001
recommended serum:ATCC 30-2020
purified DNA:ATCC HTB-121D
References: 22542: Lasfargues EY, et al. Isolation of two human tumor epithelial cell lines from solid breast carcinomas. J. Natl. Cancer Inst. 61: 967-978, 1978. PubMed: 212572
23093: Faust JB, Meeker TC. BT-483细胞Amplification and expression of the bcl-1 gene in human solid tumor cell lines. Cancer Res. 52: 2460-2463, 1992. PubMed: 1568216
